Sol.
Let the moles of Tris - HCl and Tris base be x and y respectively
As Volume of buffer = 0.5 L
Conc. of buffer = 0.25 M
So , Total moles of buffer = 0.25 × 0.5 = 0.125 mol
and , x + y = 0.125
Now , pH = 8.8 and pKa = 8.1
Using Henderson - Hasselbalch equation ,
pH = pKa + log ( moles of Tris base / moles of Tris - HCl )
8.8 = 8.1 + log ( y / x )
log ( y / x ) = 0.7
y / x = 100.7 = 5.0118
y = 5.0118 x
So , x + y = x + 5.0118 x = 0.125
6.0118 x = 0.125
x = 0.125 / 6.0118 = 0.0208 mol
and y = 5.0118 x = 5.0118 × 0.0208 = 0.1042 mol
As molar mass of Tris - HCl = 157.56 g / mol
mass of Tris - HCl = 0.0208 × 157.56 = 3.2772 g
molar mass of Tris base = 121.1 g / mol
mass of Tris base = 0.1042 × 121.1 = 12.6186 g
8. Tris buffers tris(hydroxymethyl)aminomethane) are extensively used in biochemistry. The pK, for Tris is 8.1. Mixing...
5. Tris i.e. [tris(hydroxymethyl)aminomethane) is commonly used as a buffer in bioanalytical studies. The pk of Tris is 8.1 at 20°C. (a) Calculate the pH of the Tris buffer after mixing 12.0 mL of 0.15 M of a HCl solution with 20.0 mL of a 0.20 M solution of Tris. (5 points) (b) An amount of 0.00017 mol of H+ was consumed during an enzymatic reaction. What is the pH of the buffer at the end of the reaction? (5...
4.48 The weak base colloquially known as Tris, and more precisely as tris(hydroxymethyl)aminomethane, has pK, = 8.3 at 20°C and is commonly used to produce a buffer for biochemical applications. (a) At what pH would you expect Tris to act as a buffer in a solution that has equal molar concentrations of Tris and its conjugate acid? (b) What is the pH after the addition of 3.3 mmol NaOH to 100 cm3 of a buffer solution with equal molar concentrations...
Tris buffers are commonly used in biochemistry labs because they buffer within the physiological range of 7.1 to 9.1 due to a pKa of 8.1. To demonstrate the buffering capacity of Tris buffer, your biochemistry lab teaching assistant has given you one liter of a 0.1 M Tris buffer at pH 7.4. Add 2 mL of 1M HCl to this buffer and calculate what the new pH will be.
Tris(hydroxymethyl)aminomethane (also known as “tris”) is a weak base commonly used in biochemical research in the preparation of buffers. Tris has low toxicity and a pKb of 5.92 that is appropriate for biological applications. a. In order to make tris buffer, a researcher weighed out 2.00 g of tris (molar mass of 121.14 g/mol) and reacted it with 15.00 mL of 0.800 M HCl. What is the pH of the resulting buffer? You may assume a total final volume of...
An enzyme-catalyzed reaction is carried out in a solution containing 0.2 M tris((hydroxymethyl)aminomethane (known as Tris, pK, 8.1). Shown below is the chemical structure in its protonated form. *NH, HOCH, C- CH, OH CHLOH The pH of the reaction mixture at the start is 7.8. As a result of the reaction, 0.033 moles/liter of His consumed. What will be the ratio of Tris (free base) to Tris (protonated form) at the beginning of the reaction? A B. C. D. 5:1....
8. One of the most common buffers in biochemistry and molecular biology involves tris which we will abbreviate as TNH2 (pKb=5.93). What volume of 0.300 M HCI must you add to 200.0 mL of 0.100 M TNH2 in order to create a buffer solution with pH=8.17? (12 points)
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