Question

Tris is one of the most common buffers used in biochemistry. A buffer is a molecule that helps maintain a stable pH. Applications of TRIS are plenty and range from DNA extraction to protein purification.  

a) Draw the Lewis structure of TRIS HOCHCNH (this arrangement gives a hint on the structure).

b) Describe the bond between C and H in detail (ex. 3sp3-2sp3 σ):

c) Draw the valence bond diagram of the structure and make sure to indicate σ and π bonds including the hybridized orbitals they were formed from.

d) What is the shortest bond in this molecule. Explain.  

Answer 1

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