Question

One of the most common buffers used in protein chemistry is a weak base called TRIS (the nitrogen atom of the amino group is the basic portion of the molecule). Write balanced equations for the following. Include phases in parentheses. Use "TRIS" to represent (HOCH₂)₃CNH₂. Write cations (if any) first, followed by molecules (if any), and finally anions (if any) last. For ions, put the charge in parentheses followed by phase in parentheses. If a charge is greater than one, list the multiple first followed by the sign.

1. Write a balanced equation that shows how TRIS acts as a weak base in water.

2.Buffer solutions are prepared from TRIS by adding enough 12 M HCl to produce concentrations appropriate for the desired pH. Write a balanced equation that shows what happens 12 M HCl is added to an aqueous solution of TRIS.

3.Write a balanced equation that shows what happens when hydroxide ions are added to a TRIS buffer solution.

4.Write a balanced equation that shows what happens when hydronium ions are added to a TRIS buffer solution.

Answer 1