Question

A preparation of egg white lysozyme was prepared in a buffered saline solution and measured in a spectrophotometer at a wavelength of 280 nm, in a 1 cm path length cuvette. The absorbance was measured at 0.460. The reference solution (blank) had an absorbance of 0.125.

The molar extinction coefficient (ε) at 280 nm for egg white lysozyme is approximately 36,000 M^-1cm^-1

Calculate the concentration of this solution? Select the correct unit.

(HINT: The blank is not zero, so what should be done before continuing with the calculation?)

Answer 1