Question

please help me biochemistry and molecular biology

Question 1 Following the renaturation of unfolded, denatured ribonuclease: Non-polar molecules influence the structure of the protein Disulphide cross-links correctly reformed Only a small percent of the protein was recovered The specific activity of the enzyme was determined.

Answer 1

Option [C] correct ..

renaturation is the process in which we again revert the action done during denaturation of proteins.

since during renaturation the conversion of denatured nucleic acid to its native configuration.

renaturation means reconstruction of nucleic acid to its original form

renaturation can be carried out by slowly cooling or by removing the denaturation causing factors like heat light

and nucleic acid then slowly converted from to single strands to its original double stranded helix fully sulphide cross linked as it was before denaturation .

hence option c can't be correct because 100 percent rinonucleic acid recovery takes place.

option C is appropriate because sdiulphide cross linking correcly reformed