Question

27. Draw the fatty acid indicated by the name a, 18:1 (as) cis-9-Octadecenoic acid b. 20-4 (A5,8,11,14) Arachidonic acid dsitds 忙。 c 160 Palmitic acd 28. Which of the restriction nucleases listed below can potentially cleave a segment of cONA that encodes the peptide LEASH? Pst 1 CTGCAG E =W.sere atiㅕ (1) 2- Eco R1 GAATTC 4) in Nsi 1 ATGCAT R。minw zant stip ha A+G (c)

Answer 1

27Ans)

28) Ans:Since, the aminoacid sequences that encode LEASR does not contain other recognition sequences, except Hind III, it can only be cleaved by the Hind III restriction nuclease only which has recognition sequence of AAGCTT

日勺. vib frexlsx -Excel shravani srinivas File Home Insert Page Layout Formulas Data Review View Help ChemOffice12Tell me what you want to do x fHence it can be cleaved by HIND III because it has the recorginition sequence AAGCTT 0 1 Leucine Glutamic Acid Alanine Serine Arginine Hence it can be cleaved by HIND IlI because it has the recorginition sequence A AGCTT CGT СТС CGC CGA CGG TCC CTA GCG TCG TTA AGA TTG AGG 12 13 14 15 17 19 21 Sheet1 21:44 O Type here to search ョ瘟脈44) ENG 27-03-2019

Note: Peptide can be cleaved by a restriction nuclease only when the peptide has a recognition sequence of the nuclease.