Question

Fill in the blanks regarding DNA hybridization (5 marks): a. Increasing temperature towards the Tm duplex hybridization. b. Adding organic solvent to a sample of hybridized dsDNA temperature Doubling the amount of dsDNA in a sample 1. the stringency of DNA the melting the Tm and C. the A260 value d. If A250 1.0 in a dsDNA sample under standard conditions, and then the sample is incubated at its Tm, the A2s0 Value will A260 change by? BONUS: what percentage will

Answer 1

1.

a. Increasing temperature towards the Tm (decreases) the stringency of DNA duplex hybridization.

b. Adding organic solvent to a sample of hybridized double stranded DNA (decreases) the melting temperature.

c. Doubling the amount of double stranded DNA in a sample (increases) the Tm and (increases  ) the A260 value.

d. If A260 =1, in a double standard DNA and sample is incubated at melting temperature, A260 value will (decreased ).