Question

Which one change would be most likely to decrease the deltaG of an enzyme-catalyzed reaction, according to our coverage? Use a version of the enzyme with a lower Kcat. Use a version of the enzyme with a lower Km. Find a way to remove product of the reaction (eg, by consuming that product in another reaction) Add a noncompetitive inhibitor. Double the enzyme concentration QUESTION 14 Think about an 800-amino acid polypeptide. In the middle (let's say amino acids 401-402) there is a ser linked to a Phe. Let's visualize this polypeptide and answer a few questions about it Fact: in a standard codon table, we can see that TCG corresponds to Ser. (There are also additional codons that correspond to Ser, but we'll ignore them for this question.) Don't ask whether codon tables give sequence 5 -> 3' or 3- 1 Seeing what information is contained in a codon table was part of our coverage Without additional information, which one of the following can best be identified as a true statement? Because of the sequence given in this question, an anticodon (not codon)sequence corresponding to Seri BUCGS The protein contains exactly 800 peptide bonds Because of the sequence given in this question, the portion of a DNA coding strand (not template strand) that encodes Ser has the sequence 3-TCG 3. Each peptide bond consists of a covalent linkage between an amino N and an alpha-C Ser is normally deprotonated in most proteins to form an alkoxide ion at physiological pri

Answer 1

Which one change would be most likely to decrease the deltaG of an enzyme-catalyzed reaction?

Lower Km. This increases the enzyme's efficiency to process substrate at low quantitites.

Question 14

Answer: Because of the sequence given in this question, the portion of s DNA coding strand that encodes Ser has a sequence 5'-TCG-3'.

Why? According to the question, the answer has to be obtained without any additional information. Because of this, the las two options can not be the answer, since we need aditional info to know wether or not all peptide links are covalent AND to know if Ser is protonated or deprotonated. None of this information is provided by the question.

Of the remaining options, the codon for Ser is 5'- UCG-3', the anti codon (the reverse complementary chain) would be 3'-AGC-5', which is not the option you are given. And the number of peptide links in the polypeptide is the total numer of amino acids minus 1. In this case, 800-1= 799 peptide bonds. This is because a peptide bond is between two molecules: every two amino acids have ONE peptide bond between them.