A Lab Report on Biochemical properties of gram negative organisms

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A Lab Report on Biochemical properties of gram negative organisms

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Answer 1

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Biochemical properties of gram negative organisms include;

Oxidase test-is negative for Enterobacteriaceae and positive for Pseudomonadaceae
Catalase test-is negative for gram negative.positive for Staphylococcus and Micrococcus
Sugar broth with durham tubes-All gram negative ferment glucose.and some produce gas
Methyl Red/Voges-Proskauer(MR/VP)-Escherichia coli is MR+ VP-.Enterobactor aerogens and Klebsiella pneumoniae MR- VP+.Pseudomonas aeroginosa is MR and VP negative
Indole test-is positive for E.coli
Nitrate broth-most gram negative are nitrate reducing and hence positive
Simmon's citrate agar-Klebsiella pneumoniae and Proteus mirabilus are positive.E.coli and Shigella dysententriae are negative
Urease test-used to distinguish the genus Proteus from other Enteric bacteria.Proteus mirabilus is arapid hydrolyser of urea
TSI slant-it is often used in the selective identification of enteric bacteria including Salmonella and Shigella

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