Question

i cant figure out how to do procedure 1. not sure if my answers are right or not either.

TCG. AGCTICGA Montel-/1 DNA FINGERPRINTING-LAB REEPORT 1 23 4 56 Db L Procedure #1: a.) Look at the photo and sketch of the finished and stained paternity gel - Moodle. On the drawing to the right, sketch the location of all of the DNA bands. A2 23130 bp 9416 bp 6557 bp b.) Also on the drawing to the right, above each lane, write the name of the person from whom the DNA was extracted 4361 bp 3000 bp c.) Also on the drawing to the right, above cach DNA band, write its approximate size in base pairs (bp). 2322 bp 2027 bp d.) Also on the drawing to the right, label the location of the positive and negative electrodes. 725 bp 570 bp e.) What is the name for the DNA that is run in lane #1? f) Which man was the father? Carefully explain below how you can tell: ocedure #2: Staple your simulated paper "gel" for procedure # 2 to the back of this lab report

Answer 1

I assume that you have drawn the bands and labelled the wells correctly (answer a and b).

d) Since DNA is negatively charged, it moves from the negative electrode to the positive electrode. Thus, the side with the wells is the negative, and the opposite end is the positive.

e) The DNA in the lane one is the ladder which is used to compare the sizes of the various bands obtained.

f) The dad #1 whose DNA sample is run in lane 4 is the father. We can conclude this by matching the bands of the child with those of the father and the mother. Each band in the child's sample will either correspond to the mother or the father. The top most band of the child comes from the mother, the second band comes from dad #1, the third band also comes from dad#1 and the fourth band comes from the mother. Thus, we can conclude that dad#1 is the father of the child.