The yeast artificial chromosomes can be used to clone the DNA inserts upto 100kb. The cloned DNA insert in the above scenario is only 250kb and by this it can be known that the probability of cloned DNA insert in 500 colonies obtained would be 0.94. Option 5 is correct.
You are attempting to clone a 250-kb segment of mouse DNA in a yeast artificial chromosome....
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...