An enzyme-catalyzed reaction was carried out in 5 ml of a 0.05 M phosphate buffer, pH...
An enzyme-catalyzed reaction was carried out in 5 ml of a 0.05 M phosphate buffer, pH 6.90. As a result of the reaction, 0.01 mole/liter of H+ was produced. a) What were the concentrations of each form of the buffer at the start of the reaction? b) What was the pH at the end of the reaction? c) Was this an appropriate buffer for this experiment? Why?
Homework Answers
Add Answer to:
An enzyme-catalyzed reaction was carried out in 5 ml of a 0.05 M
phosphate buffer, pH...
An enzyme-catalyzed reaction is carried out in a 100 mL solution containing 0.1 M TRIS buffer....
An enzyme-catalyzed reaction is carried out in a 100 mL solution containing 0.1 M TRIS buffer. Th pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H^+ were produced. What is the ratio of TRIS base to TRIS acid at the start of the experiment? What is the ration at the end of the experiment? What is the final PH?
An enzyme-catalyzed reaction is carried out in a solution containing 0.2 M tris((hydroxymethyl)aminomethane (known as Tris,...
An enzyme-catalyzed reaction is carried out in a solution containing 0.2 M tris((hydroxymethyl)aminomethane (known as Tris, pK, 8.1). Shown below is the chemical structure in its protonated form. *NH, HOCH, C- CH, OH CHLOH The pH of the reaction mixture at the start is 7.8. As a result of the reaction, 0.033 moles/liter of His consumed. What will be the ratio of Tris (free base) to Tris (protonated form) at the beginning of the reaction? A B. C. D. 5:1....
a) What are the concentrations of Na2HPO4 and NaH2PO4 in a 0.30 M phosphate buffer solution...
a) What are the concentrations of Na2HPO4 and NaH2PO4 in a 0.30 M phosphate buffer solution pH 7.0? Use pKa 6.82 b) Describe how you would prepare 250 mL of the buffer in part (a) given that you have available to you a 1.0 M stock solution of NaH2PO4 and solid Na2HPO4(FW 141.96 g/mol). Provide your answer in milliliters of NaH2PO4 and grams of Na2HPO4. c) Suppose you use 100 ml of this buffer in an experiment and 0.003 mol...
an enzyme-catalyzed reaction was carried out with the substrate concentration initially a thousand times greater than...
an enzyme-catalyzed reaction was carried out with the substrate concentration initially a thousand times greater than the Km for that substrate. After 9 minutes, 1% of the substrate had been converted to product, and the amount of product formed in the reaction mixture was 12micromols. If in a separate experiment, one third as much enzyme and twice as much substrate had been combined how long would it take for the same amount of product to form?
Does anyone know how to do this? Thanks in advance! Enzyme Activity (ml) Effect of pH...
Does anyone know how to do this? Thanks in advance!
Enzyme Activity (ml) Effect of pH on (amylase) enzyme activity. For determination of optimum assay pH of the enzyme reaction, 0.05 M Na2HPO4-NaH2PO4 buffer was used. The reaction was carried out for 10 min at 50°C in a shaking water. The enzyme activity was measured and the results are presented on graph, Bars represent means standard deviations for three replicates. 1. What do the error bars in this graph represent?...
12,13,14 12 Assume that an enzyme-catalysed biochemical reaction is carried out in a solution ontaining 0.2...
12,13,14
12 Assume that an enzyme-catalysed biochemical reaction is carried out in a solution ontaining 0.2 M tris(Chydroxymethylaminomethane (known as Tris, pk, 8.1). Shown below is the chemical structure in its protonated form. OH HN GOH OH The pH of the reaction mixture at the start is 8.4. As a result of the reaction, 0.033 molester of H was generated. What was the ratio of Tris (free base) to Tris (protonated form) at the beginning of the reaction? 9.45 1...
Pre-Lab Assignment Hydrogen Phosphate Buffer System This pre-lab assignment is mandatory. You will receive a zero f...
Pre-Lab Assignment Hydrogen Phosphate Buffer System This pre-lab assignment is mandatory. You will receive a zero for the experiment if the pre-lab assignment is not completed before the beginning of the lab session. 1. Calculate the masses of the conjugate acid and base needed to prepare each of the six buffer solutions listed in the table on the left. (p of H,PO,= 6.70) Assume the availability of the phosphate salts listed in the table on the right. Fill out the...
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
1) How would you make 300 ml of a 0.1 M sodium phosphate buffer, pH 7.0...
1) How would you make 300 ml of a 0.1 M sodium phosphate buffer, pH 7.0 (pKa = 7.2) phosphate dibasic (the conjugate base). 2) What are the concentrations of acetate and acetic acid in a 0.2 M acetate buffer pH 5.3? The pKa for acetic acid is 4.76. 3)You have 100 ml of 0.1 M acetate buffer pH 5.2 (pKa 4.76). You add 10 ml of 0.1 M NaOH. Calculate the resulting change in pH and the buffering capacity...
help me out, please! answer all the multiple choice. (15) A phosphate buffer solution is prepared by mixing 100. m...
help me out, please! answer all the multiple
choice.
(15) A phosphate buffer solution is prepared by mixing 100. mL. of 0.300 M KH PO, and 150.ml 0.500 M K HPO (a) Calculate the molar concentrations of H:PO, and that of HPO,2 in the buffer solution. (b) What is the pH of the buffer solution? (H,PO, has K,-6.2 x 10 (c) Write a net ionic equation for the bufering reaction against a strong acid, Hjo (d) Calculate the new concentration...
An enzyme-catalyzed reaction is carried out in a solution containing 0.2 M tris((hydroxymethyl)aminomethane (known as Tris, pK, 8.1). Shown below is the chemical structure in its protonated form. *NH, HOCH, C- CH, OH CHLOH The pH of the reaction mixture at the start is 7.8. As a result of the reaction, 0.033 moles/liter of His consumed. What will be the ratio of Tris (free base) to Tris (protonated form) at the beginning of the reaction? A B. C. D. 5:1....
Does anyone know how to do this? Thanks in advance!
Enzyme Activity (ml) Effect of pH on (amylase) enzyme activity. For determination of optimum assay pH of the enzyme reaction, 0.05 M Na2HPO4-NaH2PO4 buffer was used. The reaction was carried out for 10 min at 50°C in a shaking water. The enzyme activity was measured and the results are presented on graph, Bars represent means standard deviations for three replicates. 1. What do the error bars in this graph represent?...
12,13,14
12 Assume that an enzyme-catalysed biochemical reaction is carried out in a solution ontaining 0.2 M tris(Chydroxymethylaminomethane (known as Tris, pk, 8.1). Shown below is the chemical structure in its protonated form. OH HN GOH OH The pH of the reaction mixture at the start is 8.4. As a result of the reaction, 0.033 molester of H was generated. What was the ratio of Tris (free base) to Tris (protonated form) at the beginning of the reaction? 9.45 1...
Pre-Lab Assignment Hydrogen Phosphate Buffer System This pre-lab assignment is mandatory. You will receive a zero for the experiment if the pre-lab assignment is not completed before the beginning of the lab session. 1. Calculate the masses of the conjugate acid and base needed to prepare each of the six buffer solutions listed in the table on the left. (p of H,PO,= 6.70) Assume the availability of the phosphate salts listed in the table on the right. Fill out the...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
help me out, please! answer all the multiple
choice.
(15) A phosphate buffer solution is prepared by mixing 100. mL. of 0.300 M KH PO, and 150.ml 0.500 M K HPO (a) Calculate the molar concentrations of H:PO, and that of HPO,2 in the buffer solution. (b) What is the pH of the buffer solution? (H,PO, has K,-6.2 x 10 (c) Write a net ionic equation for the bufering reaction against a strong acid, Hjo (d) Calculate the new concentration...
Most questions answered within 3 hours.
-
Problem: Patient Fees C++
You are to write a program that computes a patient’s bill for...
asked 1 hour ago -
In a population of interest, we know that, 77% drink coffee, and
23% drink tea. Assume...
asked 1 hour ago -
Given that f(x) = e-(x-1) for x > 1, determine the following
probabilities:
a) P(X <...
asked 1 hour ago -
A mechanic pushes a 2.60 ✕ 103-kg car from rest to a speed of v,
doing...
asked 1 hour ago -
International information systems result in all of the following
except:
A. improved quality of information flow....
asked 1 hour ago -
The president of the retailer Prime Products has just approached
the company’s bank with a request...
asked 1 hour ago -
If the carrying amount is $200,000 and recoverable amount is
$205000, the impairment amount is:
Select...
asked 1 hour ago -
The correlation is inappropriate as a measure of association
between two quantitative variables (you may select...
asked 1 hour ago -
USE THE DATA IN THE TABLE BELOW TO ANSWER QUESTIONS 19 – 24
(Assume all account...
asked 1 hour ago -
Mahaley, Inc., manufactures and sells two products: Product Q9
and Product F0. Data concerning the expected...
asked 1 hour ago -
To measure the current through one branch of a parallel circuit,
the meter is connected ________....
asked 1 hour ago -
a)
The following table shows the profile, by the math section of
the SAT Reasoning Test,...
asked 2 hours ago