Question

an enzyme-catalyzed reaction was carried out with the substrate concentration initially a thousand times greater than the Km for that substrate. After 9 minutes, 1% of the substrate had been converted to product, and the amount of product formed in the reaction mixture was 12micromols. If in a separate experiment, one third as much enzyme and twice as much substrate had been combined how long would it take for the same amount of product to form?

Answer 1

27 mins.

Given, the enzyme converts 1% substrate to product (12 mmols) in 9 minutes.

Now, the enzyme is reduced to 1/3rd and substrate is increased to twice the initial amount. The level of substrte increase does not affect the conversion rate.

Since 1 enzyme converts substrate to 12 mmol product in 9 minutes, 1/3 enzyme will require 3x9 minutes i.e. 27 minutes.