Question

The rate of an enzyme-catalyzed reaction initially increases with an increase in the substrate concentration, but eventually reaches a maximum value, even though the concentration of substrate continues to increase. Which of the following best explains why? O As substrate concentration increases, the substrates preferentially bind with each other instead of the active site of the enzyme, and no additional catalysis occurs. As substrate concentration increases, the active sites of all the enzyme molecules become occupied with substrate molecules, and no additional binding can occur O An increase in substrate concentration eventually increases the temperature of the reaction mixture, and the enzyme becomes denatured. O An increase in substrate concentration allows for greater competition between substrate and inhibitor binding, and enzyme activity ceases. When substrate concentrations become too high, local variations of pH occur that cause the enzyme to become denatured

Answer 1

- Answer (E) 0 In case of Engyme caty bed Reaction. The enzyme binds with the substrate to form Enzyme-substrate CS) [es] Complex since there are limited number of Enzyme sites that react with substrate so on Increasing the substrate will Prcrease the mate Until all of Enzyme sites gets occoupied to form Enzyme a substante complex. xlow since all Enzymes got occoupied so on further increase in the substrate will not increase the late as there are no Enzymes present to form Enzyme Substrate complex. So option B As substrate concentration increases the active sites of all the enzymes becomes occoupied with substrate and no further Binding occours.

Q2. since Prreversible inhibitors Modify the Enzymes as they have certain groups that are heactive such as Aldehydes, alkenes etc. sometimes causing formation of covalent bonds, as they produce covalently modified complex. (Prreversible complex ) E+S Ž. Es -> ET Enzyme Covalently Substrate Modified compled. Compled. E= Enzyme is So this TRUE s= Substrate EI= Covalently modified complea. Q no3 In general a wax is a simple lipid - which is an ester of tong chain alcohol and an fatty acid formed by the Reaction 1. between two. - а: сн, сен, о, он + кой (см. , -4, (long chain alcohol) -H2O Cny fculpa cu insaw cho & fena) au CH3 So this is TRUE An simple upid or wax -