Question

______ 2. Using the accompanying table, determine which protein would stick to a carboxymethyl cellulose ion exchange column (the column has stationary carboxyl groups) at pH 7.0.

a) glucose oxidase      b) horse myoglobin                c) ribonuclease A       d) cytochrome C

            Protein                                     Isoelectric Point

Amyloglucosidase Glucose oxidase Trypsin inhibitor β-Lactoglobulin Carboanhydrase Myoglobin horse Lentil lectin Ribonuclease A Cytochrome C

pI 3.5 pI 4.2 pI 4.5 pI 5.15/5.3 pI 6.0 pI 7.35 pI 7.75/8.0/8.3 pI 9.45 pI 10.65

Answer 1

SOLUTION:

Ribonuclease is a protein which would stick to a carboxymethyl cellulose ion exchange column (the column has stationary carboxyl groups) at pH 7.0.