Question

How would you test the hypothesis that the C-terminal domain of a newly identified growth factor receptor is auto-inhibitory for receptor auto-phosphorylation in the absence of ligand?

Answer 1

Multicellular organism’s homeostasis and growth mainly maintained by growth factors and also tyrosine kinase receptor activity (TK). They possess extensive role in the elucidation of human cancer growth also. Cancer drugs such as Gifitinib, ematinib are the monoclonal antibodies that acts on epidermal growth factor receptors (EGFR), tyrosine kinase receptors. Inhibition of ligand dependent signaling and ligand-independent signaling of these receptors can be potentially done by these drugs.

There are characteristic dissimilarities among these even though tyrosine kinase inhibitors are analogous in that they unite within the ATP-binding region of the kinase domain.

Generally epidermal growth factor receptors kinase domain subsists in an auto-inhibited inactive conformation. Later this inactive form may take on an active conformation by asymmetric contacts through receptor dimerization.