Question

Calculate the final amount (ug) of BSA that used for standard curve. Show your calculations.

the tubes thoroughly to mix. Table Ib. Preparation of BSA Dilutions for Standard Curve. Final Final Amount of Preparation for BSA Loaded | Loading on Gel | on Gel (μg) dH20 BSA Total Conc. of Source of Volume Volume Volume BS BSA (HL) | (1) | (1) | (gjuL) 10 mg/ml stock 272 128 400 32 3.2 μguL | 200 1.6 μguL | 200 none 200 | 200 200 15 μL + 15 μL of loading dye 15 μL + 15 μL of loading dye 15 μL + 15 μL of loading dye 15 AL + 15 μL of loading dye 400 1.6 | 400 0.8 0.4 0.4 pg/ul 200 200 400 0.2 0.8 μgjuL | 200 400 0.2 μgjuL | 200 | 200 400 0.1 loading dye

Answer 1

BSA (ug/ul) Volume of solution used for loading on the gel Total amount of BSA loaded on the gel

1.6 15 ul 1.6 X 15= 24 ug

0.8 15 ul 0.8 X 15 = 12 ug  

0.4 15 ul 0.4 X 15= 6 ug

0.2 15 ul 0.2 X 15= 3 ug

0.1 15 ul 0.1 X 15 = 1.5 ug