Sketch the following proteins, including only the sorting signals, based on their FINAL DESTINATION, listed below:
A transmembrane protein (3-span ) in the plasma membrane
A soluble protein in the peroxisome (assuming cytosolic route)
A soluble ER resident protein
A 4-span membrane protein on the Plasma Membrane, with N-terminus in theextracellular space. (Where is C-terminus?)
A cytosolic protein
A membrane-bound (1-span) ER resident protein
A soluble mitochondrial matrix protein
Sketch the following proteins, including only the sorting signals, based on their FINAL DESTINATION, listed below:...
hich of the following organelles are the destination for proteins synthetized by cytosolic ribosomes Peroxisomes, endoplasmic reticulum, and the Golgi apparatus The Golgi apparatus, lysosomes, and the nucleus Peroxisomes, mitochondria, and lysosomes Peroxisomes, mitochondria, and the nucleus Mitochondria, lysosomes, and the nucleus What is the fate of N-terminus signal peptide after it directs the protein to the ER translocator O It is linked to the transported protein by S-S bond It is cleaved and then released into the membrane It...
Scientists want to design fluorescent marker proteins for different cellular compartments using the Green Fluorescent Protein (GFP). This means that the GFP will be targeted and glow at the organelle of interest and they can use these to mark different organelles during fluorescence microscopy. To make these marker proteins, they take the sequence of GFP and add the desired sorting signal. A. GFP on its own is an excellent protein to use as an organelle marker protein because it is...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence? A. This engineered GFP would not have a hydrophobic signal sequence to get it into the RER in the first place. B. The...