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After 7 days, 35% of the flies exposed to 2,5-dimethylfuran were dead. However, exposure to this...

After 7 days, 35% of the flies exposed to 2,5-dimethylfuran were dead. However, exposure to this compound did not significantly degrade the flies' performance in the geotaxis assay. What explanation could be given to explain these results?

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Answer #1

Geotaxis assay is a traditional adult climbing assay used to check the mobility of flies (behavioral asay). In this assay, the flies are put in a vial and observed how it moves & also the time taken to cross certain distance in the vial. In other words, this assay can be used check the natural tendency of the fly to move against the gravity.

volatile oragnic compound 2,5-dimethylfuran ( 2,5 DMF) ia an ether. Exposure of 2,5-dimethylfuran in flies leads to induce reactive oxygen species. Reactive oxygen species inturn cuases lipid peroxidation and results in oxidative stress induced cell death (dopaminergic neurons).

The flies may be fed with antioxidant such as vitamin E by scavenging the free radicals and thereby will not affect the performance of fly.

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