Question

1. Did the ONPG level ever fall below saturating amounts during your experiment? How can you tell if ONPG (remember, this is your substrate) was present in saturating amounts during the enzymatic reaction by looking at the graphs you created? Absorbance of Enzyme 1 Over Time 0.1 y 0.0062x+0.0008 008 0.06 0.04 2002 10 15 Time (min) Absorbance of Enzyme 2 Over Time 0.2 0.0125x+0.0028 R 0.9982 8 0.15 0.1 0.05 10 Time (min) 15 20 Reaction Rates of Enzyme 1 and Enzyme 2 0.014 E 0.012 0.01 0.008 0.006 0.004 0.002 E1 Enzyme Type

Answer 1

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