1)
Yield is given by:
In this example,
The Yield is 45%.
Fold purification is given by:
= 1500.
Thus, the fold enzyme was purified 1500 fold.
2)
Absorbance = cl = 0.4 mL/mg/cm * 2 mg/mL * 1 cm = 0.8
So, the absorbance of the protein is 0.8 or 80%
Sample Problem 1 TABLE 3-5 A Purification Table for a Hypothetical Enzyme Fraction volume (ml) Total...
Based on the results in the table, is anion exchange a good purification step, yes or no. Defend your answer. Could someone please explain how you would know if the purification worked looking at a table like this? Which values would change? Dous lloded during the way to Proteins in eine Wrn that once the non-poc b. A student is purifying an enzyme wid heyme which catályzes the Oxidation of alcohol functional groups Gising NAD as a cofactor. NADH t...
lab question 1. What is the basis of the different purification methods? 2. What are some of the factors the might have interfered with your results? 3. How might you improve the process to increase the yield and purity? lab process E. coli BL21 (DE3) cells were transformed with the pET Topo-1521 vector containing a reading frame encoding the green fluorescent protein (GFP). Cells were cultured in M9ZB media at 37°C until the absorbance at 600 nm reached 0.7, at...
Complete the purification table below for that group using the information supplied in Tables 1 & 2. You may refer to the description in your lab manual on how to calculate these parameters and account for any dilutions. Sample Activity Total (ABS/min/mL) activity (ABS/min OR U) Protein Concentration (mg/mL) Total Protein (mg) Specific activity (ABS/min/mg OR U/mg) Fold Purification RP PPE [28 marks] Table 1: Triplicate absorbance data of Group A for the catechol assay of banana PPO after 2...
Question 3. Draw the tripeptide: Val-Ser-Ala. Include a phosphoryl group on the amino acid sidechain that is most likely to undergo phosphorylation. Question 4. A protein retained on an ion exchange chromatography column is usually eluted off the column by (circle the correct answer) A. increasing the salt concentration in the buffer B. adding the protein's free ligand. C. changing the temperature of the elution buffer. D. allowing the retained protein to naturally come off the column after the non-specifically...
Based on the document below, 1. Describe the hypothesis Chaudhuri et al ids attempting to evaluate; in other words, what is the goal of this paper? Why is he writing it? 2. Does the data presented in the paper support the hypothesis stated in the introduction? Explain. 3.According to Chaudhuri, what is the potential role of thew alkaline phosphatase in the cleanup of industrial waste. CHAUDHURI et al: KINETIC BEHAVIOUR OF CALF INTESTINAL ALP WITH PNPP 8.5, 9, 9.5, 10,...