1. An argt cyst his met pro* bacterial strain is known to be lysogenic for a...
QUESTION 6 8 points Save Answer A bacterial strain with the genotype pro galt lysstrain was used as the donor to transform another strain with the genotype pro gal lys'. The genotypes of the transformed cells were as follows: Number of cells Genotype pro gals pro galthy progally pro galthy pro gals jo gally frogatys What is the order of the genes? Please explain your reasoning. Which two genes are closest to each other? How do you know? QUESTION 5...
strain (thrleut his pro pan bio). At each gene, + . (5 points) One triply auxotrophic E.coli strain (thr leur his pro pant bio*) is transforme ONA from a second triply auxotro ndicates prototrophy. - auxotrophy. After transformation, bacteria are plated on complete the master plate) and then replica plated onto minimal medium or minimal media with add nutrients as indicted in the figure below (recall that replica plating means that the positio colonies is the same in all plates)....
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
3. (7) Suppose that you have selected E. coli that can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis - cysX . You have two strains of bacteria: Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s F - cysX+ gal - his - lac - pur - trp - ton R A) What supplements would you need to add to...
all please Question 2 (1 point) ✓ Saved In Drosophila, the mutant black (b) has a black body and the wild-type (b+) has a gray body; the mutant vestigial (v) has wings that are short and crumpled compared the long wild-type wings (v+). These genes are linked and are located on the X- chromosome. A cross between a female fly and a black, vestigial winged male fly produced the following progeny: gray (b+), normal (v+) 20 gray (b+), vestigial (v)...
Title: Development of an Adhesion Assay and Characterization of an Adhesion-Deficient Mutant of Pseudomonas fluorescens RESULTS Bacterial adhesion to sand columns. In the initial column assays with soil-and-sand mixtures, P. fluorescens PfO-1 and E. coli SLH25 had the highest and lowest adhesion values, respectively. Therefore, the sand column assay was optimized to yield the greatest difference in percent adhesion for these two strains. PfO-1 bacteria grown in minimal medium to the logarithmic phase were washed and suspended in either minimal...