In the HPC or SPC i.e.abbrevated as Heterotrophic plate count or standard plate count, the same pippette is used because of same sample dilution is taken we need to obtain all the bacterial population in the dilution sample same pippette gives better removal sticked bacteria to the pippette walls also.
2) we need to check the temperature of the molten agar medium before the palting the sample innoculant as we know hetertrophic efficiency depends on the sample innoculation, type of the media used and temperature administered for the growth of the bacteria, the optimum temperature ie..35C is used for the efficient temperature for growth and withstand viability of the bacterial forms which present in the diluted samples..
After plating these are covered and incubated and colonies are developed and CFU palting efficiency is calculated after necessary incubation time.
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Check the temperature of the water bath containing the nutrient agar. Test the temperature of the...
please help me with letter B. Value Ulla ination of Living EXERCISE 3: MICROE b. Holding the flask at an angle, remove the stop- per with the fourth and fifth fingers of your oth- er hand. Heat the mouth of the flask by passing it through the flame three times (FIGURE 2a). Why is it necessary to keep the flask at an an- gle through this procedure? (a) Remove theflask. c. Remove the cover from the first dish with the...
letters C. & D. 35 EXERCISE 3: MICRORES IN THE ENVIRONMENT remove the stop- fingers of your oth- the flask by passing ames (FIGURE 2a). the flask at an an- first dish with the Buickly and neatly into the dish until a depth of approx 2b). Keep the flask dish cover, move he agar is poured. gently swirl the empty spaces: do he dish covers. eave the Petri plate + 15 minutes until (a) Remove the stopper, and flame the...
the weight if the initial product was 3.4 grams of methyl benzoate. Who |o yald foR 3.91SSg st prodoct nd product 2.14143 EXPERIMENTAL PROCEDURE SAFETY WEAR nitrile gloves for this experiment. The nitrating moture of sulfuric acidnitric acid (1:1) is a very strong acid system. Do not allow it to come in contact with your skin or clothing. It will cause acid burns if it comes in contact with your skin (and holes an your clothes). Wash any affected area...
infectious disease lab summary protocol. Questions that need to be answered are attached, aswell as the lab manuel Name: ID: Protocol summary for: (Maximum 2 pages, Total = 10 marks) 1) Describe, in a sentence or two, the main objective(s) of this lab. (1) 2) What techniques will you be using in this lab? (2) 3) How many microorganisms will you be working with in this lab? (1) 4) Over the course of this lab (1", 24, ... week), which...
Need help filling in the chart and answering the questions that go along with it. I have added the procedure and the instructions as well as the "results" that are supposed to be used to fill in the chart. Thank you! We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
For part 1 of this lab) I collected a soil sample from my campus Part 2) Tested bacteria initial viability Part 3) DNA extraction Part 4) DNA quantification by Nanodrop Part 5) Sample sequencing Part 6) PCR amplification Part 7) Gel electrophoresis Part 8) DNA sequence data analysis (sent sample to another lab) Directions for Part 3 DNA extraction are in the attached image QUESTIONS REGARDING PART 3 (DNA extraction) 1) What type of conclusions can be made from initially...
A sentence or two stating the hypothesis or hypotheses made. A sentence or two describing what was done A summary of the most important results A sentence stating the major conclusion EXPERIMENT 2: CHARACTERIZING BACTERIA ISOLATED FROM SOIL Data Tables Table 1: Gram Staining Observations Sample Colony 1 Colony 2 Colony 3 + "Normal" + "Heated" Table 2: Experiment 2b Catalase Assay Results Colony Catalase Assay Results ("Normal") Catalase Assay Results ("Heated') 1 2 3 + Characterizing Bacteria Isolated from...