Michaelis Menten equation:
This is an equation of a straight line ( y = mx + c) with slope = (KM / Vmax ) and y-intercept = (1/ Vmax)
From the given data:
Trial | [S]0 | v0 | 1/[S]0 | 1/v0 |
1 | 0.00051 | 3.00E-05 | 1960.784314 | 3.33E+04 |
2 | 0.00026 | 2.00E-05 | 3846.153846 | 5.00E+04 |
3 | 0.00013 | 1.00E-05 | 7692.307692 | 1.00E+05 |
Plot
vs
Fitting a straight line to the data points gives:
y-intercept = 1/ Vmax = 7864.9
Vmax = 0.00012
Slope = KM / Vmax = 11.833
KM = Vmax * 11.833
KM = 0.0015
For the part of the Enumentis Experiment fed nyme concentration was tired by altering the substrate...