Question

Please help with all parts... I am a bit confused and the prof is no help.

20.) A newly discovered oxygen binding protein (OBP) has been discovered. The Hill plot shown below was generated based on the oxygen binding parameters of OBP (dashed lines) and compared to hemoglobin ( solid line). 1 Yoz 1-Y02 Log Log po (a) (2 pts) Which of the two proteins has the higher oxygen affinity? (b) (2 pts) Calculate the hill coefficient for OBP. n = (c) ( 3 pts) Calculate the pso for OMP. pso = (d) (3 pts) At a pO2 = 10 torr, what is the percent saturation of OMP?

Answer 1

20.

(a) Myoglobin and haemoglobin are two oxygen binding protein which has higher affinity with oxygen.

(b). To calculate the hill Coefficient we must have to use the formula

Log(YO₂/1-YO₂) = n log po_{2 -} n log p₅₀

So first we have to calculate p₅₀ which is according to this graph,

The constant P50 is the value of pO2 that gives occupancy = 0.5,. P50 = 0.26 for this.

So, n = log(YO₂/1 - YO₂) + log p₅₀ ÷ log po₂

= log(1) + log (0.26) ÷ log(0.26)

= 1

(c). The constant P50 is the value of pO2 that gives occupancy = 0.5. P50 = 0.26 torr for this graph.

(d) . To calculate the percentage saturation we can use the formula.

Y =( po₂ / p_{50 +} po₂) ×100

_{. = (10/ 0.26+10) ×100}

_{. = 0.97 × 100}

_{. = 97 %}