Homework Answers
Genes which are located together on the chromosome will be always transformed consecutively or will show cotransformation.
Focus only on genes that have undergone cotransformation.
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Gene mapping by transformation 2. DNA from a strain of Bacillus subtilis with genotype at b...
DNA from a strain of Bacillus subtilis with genotype a+b+c+d+e+ is used to transform a strain...
DNA from a strain of Bacillus subtilis with genotype a+b+c+d+e+ is used to transform a strain with genotype a-b-c-d-e-. Pairs of genes are checked for co-transformation and the following results are obtained: Pair of genes Cotransformation and b+ no and c+ no and d+ yes and e+ yes and c+ yes and d+ no and e+ yes and d+ no and e+ yes and e+ no On the basis of these results, what is the order of the genes on...
An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain...
An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+ after 30 minutes a+ b+e+ after 40 minutes a+b+d+e+ after 50 minutes a+b+c+d+e+ after 60 minutes a+b+c+d+e+f+ Which represents the correct order of genes on this bacterial chromosomes? efabcd eabdcf...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and a live bacterial cell is: a. conjugation b. transduction c. transversion d. transformation 2. An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+...
1. Which strain of Escherichia coli has been transduced with the gene for the Shiga toxin?...
1. Which strain of Escherichia coli has been transduced with the gene for the Shiga toxin? (A-E) A E പറ B D с 2. Aeromonas hydrophila, the bacterium with this plasmid, was found growing in what organism? 3. The qach and qacEA1 genes provide resistance to what group of disinfectant?? 4. The tetA and tetR genes provide resistance to what type of antibiotics? Aeromones hydrophile plasmid R18 165.906be GC 52.53% 5.. Name the type of horizontal gene transfer diagrammed below....
After transformation, what will be the fate of DNA from the vector alone ligation reaction? A....
After transformation, what will be the fate of DNA from the vector alone ligation reaction? A. replicated by cellular enzymes B. degraded by cellular proteases C. integrated into bacterial chromosome through site specific recombination D. degraded by RecBCD E. integrated into bacterial chromosome through homologous recombination
genetics-gene mapping Question 6 1 pts You identified 3 genes (A, B and C) located in...
genetics-gene mapping
Question 6 1 pts You identified 3 genes (A, B and C) located in the third chromosome of the Arabidopsis plant. After many crosses you determine the following genetic distances: A - 30- MU - B - 20 MU -C Out of the 500 hundred three point cross progeny you have found 10 double cross plants. Is there genetic interference happening? What is the coefficient of coincidence? I = 1 - c.o.c where c.o.c. = (observed DCO)/(expected DCO)...
11. A gene is best defined as a. A segment of DNA b. Three nucleotides that...
11. A gene is best defined as a. A segment of DNA b. Three nucleotides that code for an amino acid. C. A sequence of nucleotides in DNA that codes for a functional product. d. A sequence of nucleotides in RNA that codes for a functional product. e. A transcribed unit of DNA. 12. Which of the following statements is false? a. DNA polymerase joins nucleotides in one direction only. b. The leading strand of DNA is made continuously c....
The genome of a newly discovered bacterial species (Bacillus sanfranciscus) is sequenced and found to have...
The genome of a newly discovered bacterial species (Bacillus sanfranciscus) is sequenced and found to have a circular genome of 8.7 x 106 base pairs (bp). Open reading frame (ORF) analysis indicated the presence of 7,250 ORFs that encode proteins with an average length of 360 aa. a. What is the information content of this genome (i.e. – how much information can be encoded in this length of DNA)?Since the genetic code can be considered digital in nature, convert the...
The genome of a newly discovered bacterial species (Bacillus sanfranciscus) is sequenced and found to have...
The genome of a newly discovered bacterial species (Bacillus sanfranciscus) is sequenced and found to have a circular genome of 8.7 x106base pairs (bp). Open reading frame (ORF) analysis indicated the presence of 7,250 ORFs that encode proteins with an average length of 360 aa. What is the information content of this genome (i.e.– how much information can be encoded in this length of DNA)? Since the genetic code can be considered digital in nature, convert the information content of base pairs...
1. (a) Microorganisms can be used to remove chemical pollutants from the environment. ine gene cluster...
1. (a) Microorganisms can be used to remove chemical pollutants from the environment. ine gene cluster xyl encodes several enzymes that are involved in degrading toluene, and transcription of these genes is activated when the xylS protein (expressed continuously under a constitutively active promoter p) binds to toluene. Only then can the resulting xyls/toluene complex bind to the pm promoter to activate transcription of the genes in the xyl cluster. By considering the cellular location of the enzymes that degrade...
1. Which strain of Escherichia coli has been transduced with the gene for the Shiga toxin? (A-E) A E പറ B D с 2. Aeromonas hydrophila, the bacterium with this plasmid, was found growing in what organism? 3. The qach and qacEA1 genes provide resistance to what group of disinfectant?? 4. The tetA and tetR genes provide resistance to what type of antibiotics? Aeromones hydrophile plasmid R18 165.906be GC 52.53% 5.. Name the type of horizontal gene transfer diagrammed below....
genetics-gene mapping
Question 6 1 pts You identified 3 genes (A, B and C) located in the third chromosome of the Arabidopsis plant. After many crosses you determine the following genetic distances: A - 30- MU - B - 20 MU -C Out of the 500 hundred three point cross progeny you have found 10 double cross plants. Is there genetic interference happening? What is the coefficient of coincidence? I = 1 - c.o.c where c.o.c. = (observed DCO)/(expected DCO)...
11. A gene is best defined as a. A segment of DNA b. Three nucleotides that code for an amino acid. C. A sequence of nucleotides in DNA that codes for a functional product. d. A sequence of nucleotides in RNA that codes for a functional product. e. A transcribed unit of DNA. 12. Which of the following statements is false? a. DNA polymerase joins nucleotides in one direction only. b. The leading strand of DNA is made continuously c....
1. (a) Microorganisms can be used to remove chemical pollutants from the environment. ine gene cluster xyl encodes several enzymes that are involved in degrading toluene, and transcription of these genes is activated when the xylS protein (expressed continuously under a constitutively active promoter p) binds to toluene. Only then can the resulting xyls/toluene complex bind to the pm promoter to activate transcription of the genes in the xyl cluster. By considering the cellular location of the enzymes that degrade...
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