In class we discussed four different methods of peptide fragmentation that can be used for MS/MS-based...
In class we discussed four different methods of peptide fragmentation that can be used for MS/MS-based sequencing. What are the four different methods and what types of ions do each usually produce?
Homework Answers
1. Tandem mass spectrometry: A technique in an instrumental analysis where two or more mass spectrometers are coupled together using an additional reaction step to increase their abilities to analyze chemical samples. A common use of tandem-MS is the analysis of biomolecules, such as proteins and peptides.
2. MALDI-MS: Matrix-Assisted Laser Desorption/Ionization is an ionization technique that uses a laser energy absorbing matrix to create ions from large molecules with minimal fragmentation.
3. HR-MS: High-Resolution Mass-Spectrometry features both high resolution and high mass accuracy and is a powerful tool for the analysis and quantitation of compounds, determination of elemental compositions, and identification of unknowns.
4. GPC: Gel Permeation Chromatography is a type of size exclusion chromatography (SEC), that separates analytes on the basis of size. The technique is often used for the analysis of polymers.
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In class we discussed four different methods of peptide
fragmentation that can be used for MS/MS-based...
2. In class, we discussed several ways that firms can secure financing for their strategies. Discuss...
2. In class, we discussed several ways that firms can secure financing for their strategies. Discuss the EBIT/EPS model. What are the advantages and disadvantages of each of the methods of securing funds?
Consider the four page replacement algorithms we have discussed in class: FIFO, OPR. LRU, and MFU....
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4. We have discussed in class that a monopolist can maximize its profit by charging different...
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2. Consider a circular array based Queue as we have discussed in the lectures (class definition...
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can someone help explain this for me? In the class we discussed the class Account, suppose...
can someone help explain this for me?
In the class we discussed the class Account, suppose we have a constructor accepting one number as the parameter to indicate the initial balance for the Account object. What does the computer do when it executes the following statements? What are the differences between the two statements? Try to give as complete an answer as possible. Account[] accl= new Account[30]; Account acc2-new Account (30);
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We discussed in class how ligands can affect the A. energy splitting. a. Categorize these complexes...
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2. In class, we discussed several ways that firms can secure financing for their strategies. Discuss the EBIT/EPS model. What are the advantages and disadvantages of each of the methods of securing funds?
Consider the four page replacement algorithms we have discussed in class: FIFO, OPR. LRU, and MFU. Among these, some are subject to ambiguity. That is, if you and another person tried to simulate the algorithm, and followed the idea correctly, you might end up with a different result (meaning pages loaded). For each of the four, indicate which have this problem, and which won't. Justify each
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2. Consider a circular array based Queue as we have discussed in the lectures (class definition given below for reference) public class CircArrayQueue<E> implements Queue<E private EI Q private int front-0 indicates front of queue l indicates position after end of queue private int end-0: public CircArrayQueue( public int getSize (.. public boolean isEmpty ( public void enqueue (E e)... public E dequeue ) throws EmptyQueueException... Il constructor We are interested in implementing a Stack class based on the above...
can someone help explain this for me?
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