and Q. Many metabolites are maintained at steady-state concentrations that are far from equilibrium. A comparison...
Tautomerization of enolpyruvate to pyruvate occurs in step 10 of glycolysis. If DG°’ = -46 kJ/mol, what is the ratio of pyruvate to enolpyruvate at equilibrium? Steady-State Concentrations of Glycolytic Metabolites in Erythrocytes Metabolite mM Glucose 5.0 Glucose-6-phosphate 0.083 Fructose-6-phosphate 0.014 Fructose-1,6-bisphosphate 0.031 Dihydroxyacetone phosphate 0.14 Glyceraldehyde-3-phosphate 0.019 1,3-Bisphosphoglycerate 0.001 3-Phosphoglycerate 0.12 2-Phosphoglycerate 0.030 Phosphoenolpyruvate 0.023 Pyruvate 0.051 Lactate 2.9 ATP 1.85 ADP 0.14 Pi 1.0
Below are listed some steady-state concentrations of the intermediates of glycolysis in the human erythrocyte. Very careful measurements suggest the pH of the cytosol of a "resting" human erythrocyte is about 7.25. What is the value of G for phosphofructokinase (K.,-310 @ 37 C- З 10 К; see page 484 of your text for the reaction) İn the human (T = 37 C-3 10 K) erythrocyte given these data? (Hint: I'm askingfor the value o/1G (no△C")) (6 pts) 1. glucose...
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For each of the following questions, show all work 7. Phosphofructokinase reaction The main control step of glycolysis synthesizes fructose-1,6-bisphosphate (F1,6BP) using the enzyme a) phosphofructokinase. The AG°* for this reaction is -17.2 kJ/mol. Based on this standard state free energy, which of the following is true? OKeg 1 OKeq1 O Keg 1 b) Using the AG°" for this reaction and the phosphate hydrolysis standard state free energy changes found in Table...
Question 1 - Linked Reactions (10 marks) The reaction catalysed by pyruvate kinase is: Phosphoenolpyruvate+ ADPpyruvateATP Keg 3.63 x 105 a) Calculate the AG" for this reaction. Show your working. 3 marks b) The hydrolysis of ATP has following equation: AG 30.5 kJ/mol Calculate the AG" for the following reaction: Phosphoenolpyruvate pyruvateP Show your working. 2 marks c) At 37 °C, the steady-state concentrations of phosphoenolpyruvate, ATP, and ADP have been measured to be 23 HM, 1.85 mM and 140...