Homework Answers
the answer is option DBy incorportaing an antibiotic resistance gene to the transformants,we are giving the ability for tem to resist the antibiotic and survive.So when the bacteria are kept in the antibiotic plate,only the recombinants that have confered the antibiotic resistance by succesfully taking up the gene of interest can survive.Thus the non- recombinants can be removed.This allows the selection of recombinants.
Add Answer to:
When transforming bacteria in the lab, you co-transform an antibiotic resistance gene along with your gene...
Antibiotic selection allows you to determine which bacteria contain the plasmid of interest, but do not...
Antibiotic selection allows you to determine which bacteria contain the plasmid of interest, but do not necessarily tell you if the plasmid has taken up the recombinant DNA and contains your gene of interest. Which gene, which spans the multiple cloning site (MCS), allows you to screen for plasmids that contain your insert? A. β-lactamase (bla) B. Ori C. lacZ D. X-gal
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
Assume there is a plasmid, pAGG1 that carries two genes, geneA encoding the antibiotic resistance gene...
Assume there is a plasmid, pAGG1 that carries two genes, geneA encoding the antibiotic resistance gene to select the plasmid and geneX which is your gene of interest. Each of the two genes is about 0.6kb encoding proteins of about 200 amino acids. Question: Discuss what limitations there are in mutagenizing the entire plasmid to saturate geneX, when would you select a whole gene method to mutagenize geneX versus a site directed method?
Python 3 please Bacteria and Drug Resistance If you don't take all your antibiotics when you...
Python 3 please
Bacteria and Drug Resistance If you don't take all your antibiotics when you are sick, you could be creating super drug resistant bacteria. Create a Bacteria class and run some simulations of results. Classes You will have to implement a couple of classes in this creating the classes with methods as given will help quite a bit when it comes to finishing up this program. The methods given here have to be implemented as given, however, you...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
Draw (or arrange) only the items you need from the Lab Materials Page (picture below) in...
Draw (or arrange) only the items you need from
the Lab Materials Page (picture below) in the
order you would use them to make AND screen a gene library.
You will ‘draw’ the steps that use the Lab Materials in the correct
order to create and screen a gene library which has the assigned
gene clone. You will need to add some connecting words and phrases
to make all the steps for making your library clear.
The genomic gene clone...
can you please help me with Qs 1-6 thanks ! LAB EXERCISE #6: Kirby-Bauer Disk Diffusion...
can
you please help me with Qs 1-6
thanks !
LAB EXERCISE #6: Kirby-Bauer Disk Diffusion Test for Antibiotie Sensitivity Determination The Kirby Bauer Test is an agar diffusion test that is used to determine the effectiveness of antibiotics killing various species of bacteria. Filter paper disks saturated with the antibiotic of interest are placed a Mueller-Hinton agar plate on which bacteria that has been isolated from a clinical sample has been sarcad. The antibiotic then diffuses from the disk...
lab topic 13 watch on colony in the margin of your loba characteristics observed Repeat steps...
lab topic 13
watch on colony in the margin of your loba characteristics observed Repeat steps to with two additional species You partnerid e three dillerent species Observe Color Plates 15, 17. und 18. Describe the shape ang face, and color of these bacterial species c Results Labo 1. Complete Table 13.1 at the bottom of the pagesger F 1 to describe the three bacterial cultures you observed Ahora Discussion surfact characteristics found in the lab partner barved by you...
You will know if the ant population has reached the carrying capacity (K) of the 2...
You will know if the ant population has reached the carrying capacity (K) of the 2 acre plot when __________. a) ants are no longer born b) ants no longer die c) the ant birth rate equals the death rate d) there is no longer room for ants to tunnel e) the birds start eating the ants As a 4-year old child, your BIOL 1114 instructor contracted mumps, a viral disease. Although he is now middle-aged, he is immune to...
e) Can you identify any wildtype (nonmutant) colonies in the figures? Explain your answer. f) Can...
e) Can you identify any wildtype (nonmutant) colonies in the figures? Explain your answer. f) Can you identify any standard (non-conditional) mutants in the figures? Explain your answer. g) Can you identify any conditional mutants in the figures? Explain your answer. 23°C mutant colony in which cells proliferate at the cooler, permissive temperature but fail to proliferate at the warmer, nonpermissive temperature 23°C colonies replicated onto two identical plates and incubated at two different temperatures mutagenized cells plated out in...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
Python 3 please
Bacteria and Drug Resistance If you don't take all your antibiotics when you are sick, you could be creating super drug resistant bacteria. Create a Bacteria class and run some simulations of results. Classes You will have to implement a couple of classes in this creating the classes with methods as given will help quite a bit when it comes to finishing up this program. The methods given here have to be implemented as given, however, you...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
Draw (or arrange) only the items you need from
the Lab Materials Page (picture below) in the
order you would use them to make AND screen a gene library.
You will ‘draw’ the steps that use the Lab Materials in the correct
order to create and screen a gene library which has the assigned
gene clone. You will need to add some connecting words and phrases
to make all the steps for making your library clear.
The genomic gene clone...
can
you please help me with Qs 1-6
thanks !
LAB EXERCISE #6: Kirby-Bauer Disk Diffusion Test for Antibiotie Sensitivity Determination The Kirby Bauer Test is an agar diffusion test that is used to determine the effectiveness of antibiotics killing various species of bacteria. Filter paper disks saturated with the antibiotic of interest are placed a Mueller-Hinton agar plate on which bacteria that has been isolated from a clinical sample has been sarcad. The antibiotic then diffuses from the disk...
lab topic 13
watch on colony in the margin of your loba characteristics observed Repeat steps to with two additional species You partnerid e three dillerent species Observe Color Plates 15, 17. und 18. Describe the shape ang face, and color of these bacterial species c Results Labo 1. Complete Table 13.1 at the bottom of the pagesger F 1 to describe the three bacterial cultures you observed Ahora Discussion surfact characteristics found in the lab partner barved by you...
e) Can you identify any wildtype (nonmutant) colonies in the figures? Explain your answer. f) Can you identify any standard (non-conditional) mutants in the figures? Explain your answer. g) Can you identify any conditional mutants in the figures? Explain your answer. 23°C mutant colony in which cells proliferate at the cooler, permissive temperature but fail to proliferate at the warmer, nonpermissive temperature 23°C colonies replicated onto two identical plates and incubated at two different temperatures mutagenized cells plated out in...
Most questions answered within 3 hours.
-
Hows does water affect the digestion of fats ?
asked 1 hour ago -
A buffer that contains 0.41 M of a base, B and 0.21 M of its
conjugate...
asked 1 hour ago -
Pleaaaaaaaasssssse help me please please it's more important
:((((((((((((((((((((
CASE STUDY
Nissan Company
Nissan Motor Company,...
asked 1 hour ago -
Calculate the concentration of hydronium and hyroxide ions in 1)
2.0 mol/L HNO3 2) 0.5 mol/L...
asked 1 hour ago -
In a loop-the-loop ride a car goes around a vertical, circular
loop at a constant speed....
asked 1 hour ago -
50 grams each of water (18.015 g/mol) and ethylene glycol
(C2H6O2) (62.07 g/mol) are mixed. At...
asked 1 hour ago -
The life expectancy of sport utility vehicles follows a normal
distribution with a mean of 145.8...
asked 2 hours ago -
If an object with mass of 5 kg is pushed with a force of 552 N,...
asked 1 hour ago -
Suppose your program already has a list called nums, which
contains several numbers. Write a for...
asked 1 hour ago -
A spherical shell with a finite thickness just barely floats in
water. If the shell is...
asked 1 hour ago -
Explain in detail whether the following is true or false:
Total factor productivity will rise only...
asked 2 hours ago -
A firm pays a $2.50 dividend at the end of year one
(D1), has a stock...
asked 2 hours ago