Question

. Fill out the following chart for the organisms Bacillus cereus and another for Alcaligenes faecalis The results will be reported as: color changes of the media color or organism growth (not media) • shapes of colonies amount of growth gas production • etc. . . Results Name PEA (growth) PEA (color & colony morphology) EMB (growth) EMB (color & colony morphology) Gram stain result color and cell morphology) MSA (growth) Mannitol fermentation (MA) (color of plate) Oxygen requirement (fluid thioglycellate pattern) Citrate utilization Urea utilization Hemolysis (TBA) Oxidase test

Answer 1

Name to ii) PEA ( growth) Result Bacillus Cesena ar SRL ino, growth, severely damaged by a Phenethyl alcohol (PEA) PEA NIL (Colors ecolony mosphology) (17) EMB (growth) nebo e no goowithpastelo Branco (W) IND EMB NIL (color a colony morphology) Grom stain result Gram tre, rod shaped bacilla with square ( color & cele mosphology) enda, molet.colour on groam Staisingos (vi) MSA (growth) no growth negative (ove), doen not ferment mannitol, colorhen are deep red optimum growth in presence of oxyges but can occur under an aerobic condition. (vi) mannitoi feromentation(msa) – (color of plate (viña) oxygen requirement (fluid thioglyconate pattern (ix) Cirate utilization (*) urra utallization Hemolyan 8%) oxidase tent positive (Avel positive (tre) (*: 2 positive (tv) Negative (ove) CS Scanned with CamScanner

Resmin 2. к.) . . lored at a Alcaligenen face came ст) OGC uro 1 i*) Eon (old Name - слух и & PEA (growth) No growth PEA xx ols yoshuls) NL (colors (ND EMB (growing growth color & colony morphology) white colonial and non-pigmented ( Croam stain Слос" ~ ~ ~ Ash, vod-Shape 4 Pink colour colonien on goom Star (hi) MSA growth no growth (*) » one 1 Сексол. КРn (nse) — NTC C-vy (Colos Color of plate) tyle с Ay Per cole Ако wity) оххо судєгсбатал да кодтал ox830 е, % от Us • ro+ (o 6 5 «-(+vy (x) urra utilization -ve. (ner++) Anоlалул үo Svette) oxidase tent (osihva. (+) (13) (1) CS Scanned with CamScanner