Question

This is a question that tests your ability to apply your lab math skills When you order primers, they are delivered as dried (lyophilized) DNA in a tube. In other words, your primers arrive as a solid. To use your primers in PCR, you must resuspend these primers in water for buffer) to achieve an appropriate concentration of DNA in solution. Typically, the primer tube simply lists the mass of DNA that is in each tube. Imagine that you have just received your lyophilized primer. You read the tube, and it indicates that there are 132 micrograms of your primer present. You know that your primer is single-stranded, and is 20 nucleotides in length. Also, you know that the MW of one nucleotide s-323 g/mole. Based on this information, calculate the amount of water that you should add to create a 100 micromolar primer stock solution Paragraph B IVAA. I. E a XX, EE m- of 12pt

Answer 1

For this, you need to use the molarity equation. I suggest you to use the Molar(M) and Liter (L) units and then transform it into a better unit. Grams M= Volume x MW Volume = This is what you need to calculate M = 100 micromolar = 1x10-4M MW = 20 x 323 g/mole = 6460 g/mole Grams = 0.000132 micrograms MX MW XV = Grams 1x10-4 M x 6460 g/mole x V = 0.000132 grams 0.646 XV = 0.000132 grams 0.000132 V = = 0.000204 L = 204 microliter 0.646 So, you need to resuspend 132 micrograms of primer in 204 microliters of water