Match the terms from list A with the most appropriate terms from list B. Write in...
Match the terms from list A with the most appropriate terms from list B. Write in the appropriate letter (from list B) after each number in the space provided. You can use a letter only once and not all from list B may be used.
LIST A LIST B
cycloheximide ______ A. contains many modified and non-Watson Crick bases
Tetracycline _______ B. inhibits translocation of the ribosome
Formyl-met_________ C. inhibits peptidyl transferase reaction
Aminoacyl tRNA ______ D. initiator tRNA in prokaryotes
Chloramphenicol _____ E. inhibits aminoacyl-tRNA binding at the A site
F. site of peptidyl transferase reaction
G. Stops elongation after 1st peptide bond formation
Homework Answers
cycloheximide = B. inhibits translocation of the ribosome
Tetracycline = E. inhibits aminoacyl-tRNA binding at the A site
Formyl-met = D. initiator tRNA in prokaryotes C. inhibits peptidyl transferase reaction
Aminoacyl tRNA = A. contains many modified and non-Watson Crick bases
Chloramphenicol = F. site of peptidyl transferase reaction
Cycloheximide blocks the movement of the mRNA and two tRNA molecules associated with the ribosome during the process of translation, thereby blocking the process of translocation.
Tetracycline is an antibiotic that can bind to the small ribosomal subunit (30S). As a result, it blocks the A site of the ribosome and prevents the amino-acyl tRNA from binding.
Formyl-met is Formylated methionine which is the first amino acid used (AUG) during the initiation of translation in prokaryotes.
Aminoacyl-tRNA is basically transfer RNA charged with an amino acid. The tRNA has several unusual nucleotide bases like pseudouridine, dihydrouridine, etc.
Chloramphenicol binds and inactivates the peptidyl transferase centre of the large subunit of the ribosome and thus prevents peptide bond formation.
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