Question

In eukaryotes, genomic DNA is wrapped around the histone complex to make chromatin. The direct contacts between DNA and the histone beads interfere bindings of the transcriptional factor proteins to their cognate sequence elements, decreasing gene activities. To increase transcriptional activity of the affected genes, the RSC/CRC complex remodels chromatins such that the ________ sequences can be amenable to formation of the transcriptional initiation complex.

Select one:

a. TBP-binding and TAP-binding

b. Shine-Dalgarno

c. start codons

d. All of these

e. None of these

Answer 1

Answer : The correct option is A (TBP- binding and TAP binding)

Explanation : RSC complex (Remodeling the Structure of Chromatin) plays an important role in altering the structure of chromatin in eukaryotic systems (which in turn regulates gene expression) which provides accessability for the transcriptional machinery to carry out transcription. Once the chromatin structure is remodeled or altered, TATA binding protein (TBP) recognizes the TATA box in the promoter region of eukaryotic genes.

TBP, one of the general transcription factor are known to bind to the specific DNA sequences called TATA box (Hogness box). Upon binding, they recruits activators or respressors as well as RNA polymerase which is required for the initiation of transcription in eukaryotes.