E. coli can be found in a pond. If very high amounts of
tryptophan are present in this environment, then which of the
following conditions would likely be occurring in E. coli?
Choose YES if that condition would likely be occurring, or NO if
that condition would not likely be occurring.
Yes No The first enzyme (or enzyme complex) in the
biosynthetic pathway for tryptophan synthesis is inhibited by
feedback inhibition.
Yes No The leader-attenuator region of the trp
mRNA forms hairpins between regions 1 and 4.
Yes No TrpR is bound to allolactose.
Yes No TrpR becomes an active repressor that binds to
the operator with lower affinity than the aporepressor form.
Yes No RNA polymerase synthesizes and releases an RNA
strand about 139 ribonucleotides long.
Yes No Ribosomes translating the leader peptide does not
stall.
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Yes- If large amounts of tryptophan is present in the environment then the first enzyme involved in the synthesis of tryptophan is blocked by feedback allosteric inhibition by tryptophan.
No- The leader sequence forms a transcription termination hairpin between 1 and 2 or 3 and 4 region and not 1 and 4 region.
No- The repressor is not bound to allolactose.
No- The excess tryptophan binds to the repressor TrpR and becomes a holorepressor that binds to the operator more strongly than the aporepresor and prevents the expression of enzymes involved in the biosynthesis of tryptophan.
Yes- RNA pol transcribes and releases the leader sequence TrpL
No- Ribosomes stall due to the Formation of the termination hairpin loop in the leader sequence.
E. coli can be found in a pond. If very high amounts of tryptophan are present...