Question 1.
A restriction enzyme is a nuclease enzyme that cuts DNA sequence into fragments after recognizing specific nucleotide sequences known as recognition or restriction site.
Question 2.
RFLPs based molecular markers are amongst the first generation molecular markers used for many animal and plant genome mapping projects. This technique is based on digesting genomic DNA with certain restriction enzymes.
# full name of RFLP is Restriction fragment length polymorphisms.
# Application:
Question 3.
Matching of DNA fragments obtained from 2 different sources os called DNA fingerprinting.
# Application:
Question 4.
Polymerase chain reaction (PCR) is a technique in molecular biology to amplify single or few copies of DNA, generating millions of copies of a particular DNA sequence.
# full name of PCR is polymerase chain reaction
# Application:
# rest of questions I need more information.
Lab 8-9 Restriction Enzymes, Gel Electrophoresis, PCR 1. Introduction: Define the following concepts and processes (use...
Questions? BIOTECHNOLOGY: Describe and explain what restriction enzymes are? what their important characteristic are, and how they work, and how they can be used for forensic applications( RFLP analysis) Explain and understand gel electrophoresis, how does IT sort Dna by size? You should understand what PCR is and the basic of how IT works and its forensic applications (STR analysis) of present with a gel fragment pattern, you should be able to interpret it.
Use the lab manual to answer the following questions in COMPLETE SENTENCES. 1.Read through the introduction. Answer the questions in between each historical section. 2. Describe tge activity that you ate doung for investygation 3. What does lysis and protease mean? 3. Which disease is describe in investigation 4? what mutation? PRE-LAB: LAB EXERCISE 7 Directions: Use the lab manual to answer the following questions in COMPLETE SENTENCES AND IN YOUR OWN WORDS. Use your own handwriting, do NOT type!...