Assume you are working to sequence the genome of a newly identified alien species. The alien DNA is composed of the same nucleotides are in us and, its genome is 2-3 times larger than that of any terrestrial organism, and this genome is contained within 2 huge linear chromosomes and once smaller circular chromosome. Assume , mechanisms of biology (i.e. transcription, translation, replication, etc. are similar to those acting in terrestrial eukaryotes. That said, here is no reason to believe that the sequences/ organization of the alien genome share much in common with any of the species on earth. The problem with getting the entire alien genome sequenced using whole genome shotgun sequencing, followed by bioinformatic assembly is:
Question options:
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a). The alien genome is too big to get a full genome sequence. |
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b). Alien centromere and telomere sequences show no similarity to the sequences of such elements in terrestrial organisms |
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c). The potential presence of repetitive DNA sequences in the alien genome will make it nearly impossible to get alignments that would establish a complete, contiguous sequence of each alien chromosome. |
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d). You might not have appropriate DNA vectors to subclone pieces of the alien genome. |
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e). The one circular chromosome and the small number of linear chromosomes will present problems when performing the bioinformatic assembly. |
Option E is the most correct answer.
Option A is not correct because size of the genome has no major impact on its sequencing.
Option B is not correct because centromere and teli mere sequence should not must match to that of the humans in order to get sequence.
Option C is not correct because in human genomes there are many tandan repeats too which has been already sequence so there should not be any problem raising due to this.
Option D is not correct because vectors can be synthesized as per need like plasmids.
Option E is correct because the algorithm of computer used can't read the circular chromosome during bioinformatics assembly through the given technique of sequencing genome.
Assume you are working to sequence the genome of a newly identified alien species. The alien...
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