Question

3. a. was The analysis of the complex formed between Cu(I) and 1,10-phenanthroline conducted using molecular spectroscopic method. The results showed that this complex has a molar absorptivity (€) of 7000 L cm'mol and maximum wavelength absorption (1) of 435 nm. Calculate the absorbance of a 6.77 x 10 M solution of the complex when measured in a 1.00 cm cell at wavelength of 435 nm. b. In ultraviolet-visible (UV-Vis) spectroscopic technique, blank correction is implemented in order to reduce errors due to non-specific radiation losses (e.g. scattering, reflection) and differences between sample cuvettes. With the aid of diagrams, deduce the calculations to determine the true absorbance after performing blank correction 2 YAB2063 iil Sketch the schematic diagrams to show the implementation of blank correction in the instrumentation arrangement of single beam UV-Vis spectroscopy and double beam UV-Vis spectroscopy (identify all the components). Explain the difference of blank correction procedures between single beam UV-Vis spectroscopy and double beam UV-Vis spectroscopy

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Answer 1

by colure , Absondoonee obtained measuring A rotating dise mirror slit light source detector and mirseer 3a, Lambert-Beer's law states that there is a linear relationship between the concentration and the absorbance of the solution, which enables the concentration ik of a solution to be calculated breasuring absorbance A=EU A = Absodbanice, Es molas extinction co-efficient e: concentration ) (M4 cm") ..A - 7000 Lom mott e = path length com XG: 114 Isºl dxlcan 0.4739 0.4739 3.) i comeeted absorbance = absorbance blank absorbence A blank is the sample that contains everything except for the analyte of interest. It is just the solvent measured in uv. vis spectrometer. The absošbance blank is then substracted from the force value of Sample absorbance to get competed absorbance value deducting all seattering and reflection effects. >0 sample light m) Single Becom Spectrophoretin It perform blank correction and sample measurements using only the sample beari. Letly reference Eustonument record the interity of sample beam during blank connection (Solanu) . Then during sample measurement, the intrumet recondo intersity of sample beam (Smear). % T- Smears elette - Beam speetoo phometer → Single ugle Sample compartment Double - Beam Speetoplonetes It perform blank committen and measure samples using reference During blank correction, the instrument recordo the US dank / Rblank ratio, based on the sample beam intensity (Sblank) and the reference beam invening (Rbcany). During sample measurement; thue lodemument seconds smears/ mears on the sample beam intersing (Smears) and the reference beam intensity (Rimeare) Smears AT = R mears It measure samples assuming the intersity of Sb blank light is always constant. Rblank computer chart recorder X 100 blank cell A Hale source monoclomator Detector R both sample and S beams. ratio, based X 100 the lig source