Question

koa AB Top of Resolvine 200 116 31 22 14 WellA: MW (Protein Ladder). Well B: Sample Protein 1. Well : Sample Protein 2. Dye front Activities: Based on the picture of the destained SDS-PAGE gel, complete the following Information: -Migration distance of dye front: mm. Molecular Weight Log (MW) Distance travelled Ri (Da) of protein (mm) ladder standard 200 116 97 66 55 37 31 22 14 6 4 Unknow Samples: Protein Distance travelled Rf Log (MW) Molecular Weight (mm) (Da) Sample Sample 2

this is whst i have so its mire spread out. im not sure what else to add.. whst should i be looking for?

Answer 1

Migration distance of dye front: 75 mm

Mol. wt of ladder	log MW	Distance travelled (mm)	Rf
200	2.3010	3	3/75 = 0.04
116	2.0644	10	10/75 = 0.133
97	1.9867	12	12/75 = 0.16
66	1.8195	18	18/75 = 0.24
55	1.7404	22	22/75 = 0.293
37	1.5682	35	35/75 = 0.467
31	1.4914	38	38/75 = 0.506
22	1.3424	47	47/75 =0.627
14	1.1461	53	53/75 = 0.707
6	0.7781	63	63/75 = 0.84
4	0.6020	69	69/75 = 0.92

2.5 2 1.5 Log Mw 1 y = -1.758x + 2.319 R = 0.979 0.5 0 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 RF

Protein	Distance travelled (mm)	Rf	log MW	Mol. Wt
Sample 1	40	40/75 = 0.533	y = -1.758x + 2.319 log MW = -1.758 (0.533) + 2.319 = - 0.937 + 2.319 = 1.382	antlog. 1.382 = 24.1 Daltons
Sample 2	27	27/75 = 0.36	y = -1.758x + 2.319 Log MW= -1.758 (0.36) + 2.319 = - 0.6329 + 2.319 = 1.6861	antlog. 1.6861 = 48.54 Daltons

Thank you