- I believed the inhibited protein was ATP synthase, however the data on Figure 2 does not support this. Please help!
Answer:
Part:-1
Pyruvate + Malate:
ADP:
DNP:
Succinate:
a):
b):
1): ADP addition doesn’t drop O2 consumption as it should (meaning that something (i.e F1 complex) that binds to ADP could be different).
2): DNP also doesn’t drop the O2 consumption as it should (meaning that the proton gradient in-between mitochondrial membranes is maintained).
3): The addition of succinate to the TCAc only produces NADH (and CO2).
Part 2:
a):
b):
c):
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- I believed the inhibited protein was ATP synthase, however the data on Figure 2 does...
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