Question

Ado NINN SNITUS OR DO ADUENE ONINDOW yok Question: How will diffusion be affected by differences in surface area to volume ratio? Independent and dependent variables Independent variables the Experiment: Hypothesis: 1800 higher the diffusion effectuerness the cell will have The larger the surface/ volume rates the Prediction: Smaller cells will be more effective difussing molecules than large cells. is the of surface area/volume ratio Control and experimental groups and dependent variabe average time for diffunon 001 Procedure: A list of steps briefly describing the procedure. Analyze Results: Table 2: Amount of time for diffusion to occur in different sizes of agar cubes. Data from an actual lab has been provided for you. Average Time Side Length of cubc (mm) Surface Area /Volume Ratio Time-Trial 1 Time-Trial 2 Time-Trial 3 (min) (min) (min) 5.2 4.8 5.1 7 10.8 11.3 11.5 150 .211.2 16.2 50,3 3.5 5. 17.5 10 10 16.4 15.9 16.2 15 51 52 48 20

Answer 1

Remember: Paramecium cells have 0,33 mm length and yeast cells have 0,01mm length (i.e. there’s a great difference in surface/volume ratio)

Procedure:

Raise paramecium cells (Paramecium sp.) and yeast cells (Saccharomyces cerevisiae) and take an aliquot to ensure cells you have a good (big) number of cells in your sample.

Incubate each of the cell samples with a colorant substance (like methylene blue) for the same amount of time (like 1 min).

Afterwards wash the colorant with buffer solution and check in the microscope the colour of the paramecium and yeast cells to see how much is it coloured.

Notes:

With this procedure you can’t measure (with measuring instruments) how much is it the diffusion related to surface/volume ratio, but you can observe it by yourself!

Also, here's the graph for your table data:

Amout of time it takes for diffusion to occur according to agar blocks of different sizes 60 50 40 Average time (min) 30 20 10 0 3.5 10 5 7.5 Surface/Volume ratio