Question

Word bank:

cDNA

Cre

CRISPR

FISH

LoxP

Microarray

RFLP

RNAi

Yeast Two-Hybrid

1) This enzyme binds loxP sites and catalyzes a recombination event.

2) Two 13-bp inverted repeats separated by an 8-bp spacer that can be used to recombine DNA in vivo in transgenic organisms.

3) A gene system using Gal4 and Gal80 to detect protein-protein interactions.

4) This technique allows you to fluorescently tag certain proteins and see them in whole tissue to determine the pattern of expression.

5) A type of DNA analysis that digests DNA and looks for altered patterns of DNA fragments based on the loss of a DNA restriction site.

6) A screen using siRNAs to knock down expression of genes in humans.

7) A library in which clones reveal which parts of the genome contain the information used in making proteins in specific tissues.

8) This technique can be used to simultaneously search for thousands of SNPs by using a cDNA probe hybridized to multiple fragments of DNA containing the SNPs.

Answer 1

1) Cre recombinase
This enzyme binds loxP sites and catalyzes a recombination event.

2) LoxP (locus of X-over P1)
Two 13-bp inverted repeats separated by an 8-bp spacer that can be used to recombine DNA in vivo in transgenic organisms

3) Yeast Two-Hybrid Assay
A gene system using Gal4 and Gal80 to detect protein-protein interactions.

4) FISH (Fluorescent InSitu Hybridization)
This technique allows you to fluorescently tag certain proteins and see them in whole tissue to determine the pattern of expression.

5) RFLP (Restriction Fragment Length Polymorphism)
A type of DNA analysis that digests DNA and looks for altered patterns of DNA fragments based on the loss of a DNA restriction site.

6) RNAi (RNA interference)
A screen using siRNAs to knockdown expression of genes in humans.

7) cDNA
A library in which clones reveal which parts of the genome contain the information used in making proteins in specific tissues.

8) Microarray
This technique can be used to simultaneously search for thousands of SNPs by using a cDNA probe hybridized to multiple fragments of DNA containing the SNPs.