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Why is the chemical makeup of a complex media unknown? Differential media agar plates often change color. What is the color c
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1 Ans: Chemical composition of complex media unknown-

Complex media provide a full range of growth factors that can support the need of unknown bacteria. It contains blood, serum, tissue extract, beef or yeast extact, peptone, glucose, agar and water.

2 Ans: Color change aids in distinguishing bacteria.

For example- A colored halo is formed when S. aureus is grown on differential media as its colonies ferment mannitol and produce acid that reacts with indicator dye of the medium.

3 Ans: If color indicator will not be added then it will be impossible to distinguish bacteria based on visible traits like color change. Other techhniques like 16s r RNA sequencing must be adoped for the identification of bacterial strains.

4 Ans: No, microbes will behave exactly the same.

For example, S. aureus will grow, erment mannitol and produce acid but acid will not react will any indicator and thus, no halo will be observed.

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