Question

In the lab, you have a strain that is normally an auxotroph. You conduct a mutagenesis on this strain to try and 'rescue' that phenotype (make it no longer an auxotroph). How would you go about selecting for mutant strains that are no longer auxotrophs?

a) Add in a compound (like an antibiotic) to kill the bacteria that did not mutate.

b) Remove the key nutrient from the growth medium.

c) By using transduction.

Answer 1

b) Remove the key mibient from the growth medium An acuxoboph is an organism that cannot synthesize a particular organic compound required for its growth of this, resuires this particular compound to be provided externally for its growth & development Thus, in order to select for mutant strains that too longer auxotrophis when key ing mitrient remored the auxotrophis are killed. And this results in selecting the non auxotrophes mit mutant strains the are no is automatically

Felt great to answer. Good luck!