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Part C. Quantitative Spectroscopy Wavelength(A) Absorbance Amax-10 Amax 10 Wavelength used for Analysis in Part C:...
I am stuck on how to find concentration for standards and unknowns. Any kind of help would be appreciated. The correct...
I am stuck on how to find concentration for standards and
unknowns. Any kind of help would be appreciated.
The corrected absorbance is = Blank - absorbance
1. Prepare the BCA working reagent. Use a graduated cylinder and add 30 ml of the BCA reagent into a 50 ml Erlenmeyer flask. Use the appropriate micropipette to add 600 uL of the CuSO4 solution into the flask and mix by swirling the flask. Record the exact mass of albumin and the...
question one question two Aspirin lab (10 points) 5. a) Spectroscopy is being used to determine...
question one
question two
Aspirin lab (10 points) 5. a) Spectroscopy is being used to determine the concentration of aspirin in an unknown sample. The absorbance spectrum of the highest concentration standard [Fe3+, SA) prepared is given below. What wavelength should be used to create the calibration curve for the experiment? Explain your reasoning. 0.14 Absorbance 0.02 250 300 450 500 350 400 Wavelength (nm) Wavelength Explain your reason briefly. b) In this experiment an aspirin tablet was placed in...
PROBLEM: Predict the molar absorptivity value of copper sulfate pentahydrate at 380 nm. Here's what I...
PROBLEM: Predict the molar absorptivity value of copper
sulfate pentahydrate at 380 nm.
Here's what I think I have to do:
I think I need to use beer's law. Abs=ebc; e=Abs/bc.
b is 1.66 cm. I think c is .400 M NiSO4, but I'm not sure. I
also think Abs is 0.758 given the graph. (If wavelength is 380 nm,
then it should be between 0.598 and 0.798, right?) My answer is
1.14 L/mol*cm, but not sure if I am...
PROCEDURE A. ABSORBANCE SPECTRA 1. Obtain three cuvettes. Add-2mL of the following samples to them: red...
PROCEDURE A. ABSORBANCE SPECTRA 1. Obtain three cuvettes. Add-2mL of the following samples to them: red dye, blue dye, yellow dye. 2. Obtain a spectrum for each dye according to the provided directions (water is the blank). 3. For each of the three dyes (red, yellow, and blue) record the wavelength for the largest peak in the visible range (380 nm to 750 nm), Record this in the data table below. Save the red dye for part B. 4. Identify...
Name: Determination of Solution Concentration by Spectrophotometry PRELAB QUESTIONS 1. A student measures the absorbance of...
Name: Determination of Solution Concentration by Spectrophotometry PRELAB QUESTIONS 1. A student measures the absorbance of an unknown after placing a sample in a cuvette which contained some water. How will this affect the concentration he reports? 2. Explain why it is all right to leave water in the volumetric flask before you put in the 10.00 mL of your unknown. 3. A 1.3 x 10-M solution of an unknown substance has an absorbance of 0.345 at its max. a)...
please help asap and the values are from the procedure there is no additional info QUESTION. PROCEDURE 1. Obtai...
please help asap and the values are from the procedure there is no
additional info
QUESTION.
PROCEDURE 1. Obtain seven spectrophotometer tubes, three 5 mL pipets and seven 125 mL Erlenmeyer flasks. One for the blank and six sample test tubes. Make sure to label the test tubes with appropriated calculated concentration. Label one flask as NaSCN 2. Pipet 5 mL of 0.00020 M NaSCN into Erlenmeyer flask #1. Pipet 5 mL of 0.20 M Fe(NO3)3 into Erlenmeyer flask #1....
You prepared standard benzoic acid and caffeine solutions that allowed you to produce four standard curves...
You prepared standard benzoic acid and caffeine solutions that allowed you to produce four standard curves with the linear equations listed below. You also diluted an unknown solution by taking a 4 mL aliquot with a volumetric pipet and transferring it into a 100 mL volumetric flask that contained 10 mL of 0.10 M HCl. You then diluted it to the mark with Dl water. You measured the absorbance at both wavelengths for the diluted unknown. The data is shown...
these are my absorbance numbers after dilution. How do i figure out my plots using beers...
these are my absorbance numbers after dilution.
How do i figure out my plots using beers law? NOT USING EXCEL Im
required to use the graph which is in the last picture.
*please read the lab or dont answer* thank you!
Amax- Procedure: Part B: Beer's Law 1. Wash, rinse and dry four medium-sized test tubes. 2. Into the first test tube, pour 10 mL of the stock solution. 3. Into tube #2, pipet 5 mL of H20 and 5...
solve all 3 dont waste my answer 1. Check Beer's Law by plotting absorbance vs molar concentration using 2....
solve all 3 dont waste my answer
1. Check Beer's Law by plotting absorbance vs molar concentration using 2. Is Beer's Law valid for this system? Explain your answer. 3. If Beer's Law is valid, the value of "E" should be constant. Use A-ECPL or E = A/(EL) and the data from Table II to calculate values of E for the five solutions below. The value of L is normally 1 cm so E - A/C in this experiment. Table...
C. Problems 1Explain the difference between transmittance, absorbance, and molar absorptivity Which one is proporti...
C. Problems 1Explain the difference between transmittance, absorbance, and molar absorptivity Which one is proportional to concentration? 2. The absorbance of a 2.3lx 10 M solution of a compound is 0.822 at a wavelength of 266 nm in a 1.00 cm cell. Calculate the molar absorptivity at 266 nm 3. A compound with a molecular weight of 292.16 was dissolved in a 5-ml volumetric flask. A 1.00-mLaliquat was withdrawn, placed in a 10-mL volumetric flask, and diluted to the mark....
I am stuck on how to find concentration for standards and
unknowns. Any kind of help would be appreciated.
The corrected absorbance is = Blank - absorbance
1. Prepare the BCA working reagent. Use a graduated cylinder and add 30 ml of the BCA reagent into a 50 ml Erlenmeyer flask. Use the appropriate micropipette to add 600 uL of the CuSO4 solution into the flask and mix by swirling the flask. Record the exact mass of albumin and the...
question one
question two
Aspirin lab (10 points) 5. a) Spectroscopy is being used to determine the concentration of aspirin in an unknown sample. The absorbance spectrum of the highest concentration standard [Fe3+, SA) prepared is given below. What wavelength should be used to create the calibration curve for the experiment? Explain your reasoning. 0.14 Absorbance 0.02 250 300 450 500 350 400 Wavelength (nm) Wavelength Explain your reason briefly. b) In this experiment an aspirin tablet was placed in...
PROBLEM: Predict the molar absorptivity value of copper
sulfate pentahydrate at 380 nm.
Here's what I think I have to do:
I think I need to use beer's law. Abs=ebc; e=Abs/bc.
b is 1.66 cm. I think c is .400 M NiSO4, but I'm not sure. I
also think Abs is 0.758 given the graph. (If wavelength is 380 nm,
then it should be between 0.598 and 0.798, right?) My answer is
1.14 L/mol*cm, but not sure if I am...
PROCEDURE A. ABSORBANCE SPECTRA 1. Obtain three cuvettes. Add-2mL of the following samples to them: red dye, blue dye, yellow dye. 2. Obtain a spectrum for each dye according to the provided directions (water is the blank). 3. For each of the three dyes (red, yellow, and blue) record the wavelength for the largest peak in the visible range (380 nm to 750 nm), Record this in the data table below. Save the red dye for part B. 4. Identify...
Name: Determination of Solution Concentration by Spectrophotometry PRELAB QUESTIONS 1. A student measures the absorbance of an unknown after placing a sample in a cuvette which contained some water. How will this affect the concentration he reports? 2. Explain why it is all right to leave water in the volumetric flask before you put in the 10.00 mL of your unknown. 3. A 1.3 x 10-M solution of an unknown substance has an absorbance of 0.345 at its max. a)...
please help asap and the values are from the procedure there is no
additional info
QUESTION.
PROCEDURE 1. Obtain seven spectrophotometer tubes, three 5 mL pipets and seven 125 mL Erlenmeyer flasks. One for the blank and six sample test tubes. Make sure to label the test tubes with appropriated calculated concentration. Label one flask as NaSCN 2. Pipet 5 mL of 0.00020 M NaSCN into Erlenmeyer flask #1. Pipet 5 mL of 0.20 M Fe(NO3)3 into Erlenmeyer flask #1....
You prepared standard benzoic acid and caffeine solutions that allowed you to produce four standard curves with the linear equations listed below. You also diluted an unknown solution by taking a 4 mL aliquot with a volumetric pipet and transferring it into a 100 mL volumetric flask that contained 10 mL of 0.10 M HCl. You then diluted it to the mark with Dl water. You measured the absorbance at both wavelengths for the diluted unknown. The data is shown...
these are my absorbance numbers after dilution.
How do i figure out my plots using beers law? NOT USING EXCEL Im
required to use the graph which is in the last picture.
*please read the lab or dont answer* thank you!
Amax- Procedure: Part B: Beer's Law 1. Wash, rinse and dry four medium-sized test tubes. 2. Into the first test tube, pour 10 mL of the stock solution. 3. Into tube #2, pipet 5 mL of H20 and 5...
solve all 3 dont waste my answer
1. Check Beer's Law by plotting absorbance vs molar concentration using 2. Is Beer's Law valid for this system? Explain your answer. 3. If Beer's Law is valid, the value of "E" should be constant. Use A-ECPL or E = A/(EL) and the data from Table II to calculate values of E for the five solutions below. The value of L is normally 1 cm so E - A/C in this experiment. Table...
C. Problems 1Explain the difference between transmittance, absorbance, and molar absorptivity Which one is proportional to concentration? 2. The absorbance of a 2.3lx 10 M solution of a compound is 0.822 at a wavelength of 266 nm in a 1.00 cm cell. Calculate the molar absorptivity at 266 nm 3. A compound with a molecular weight of 292.16 was dissolved in a 5-ml volumetric flask. A 1.00-mLaliquat was withdrawn, placed in a 10-mL volumetric flask, and diluted to the mark....
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