Describe why Michaelis constant (km) and the dissociation constant (Kd) should not be considered to be...
Describe why Michaelis constant (km) and the dissociation constant (Kd) should not be considered to be the same. Use the rate constant definitions of these terms in your explanation, and also indicate in what case Km & Kd can be considered to be the same?
Homework Answers
Add Answer to:
Describe why Michaelis constant (km) and the dissociation
constant (Kd) should not be considered to be...
The Michaelis and Menten constant, Km is actually a summary of 3 terms:
4.4 For the enzyme reaction:The Michaelis and Menten constant, Km is actually a summary of 3 terms:(a) What are these? (b) What assumption does it make?(c) Define and calculate Km from the above equation given:(d) Which is the rate-limiting step? And why?
A single-substrate enzyme that obeys Michaelis-Menten kinetics displays the following parameters: Kd = 3.1 x 10^-5...
A single-substrate enzyme that obeys Michaelis-Menten kinetics displays the following parameters: Kd = 3.1 x 10^-5 M; k1 = 107 M-1 s-1, and kcat = 10^4 s-1. Given this information, what is the value of the Michaelis constant Km? Express your answer in terms of mM to four significant figures.
The Km can be considered to be the same as the dissociation constant K for E+...
The Km can be considered to be the same as the dissociation constant K for E+ S binding it: the concentration of [ES] is unchanged k1 >> K2 ES --> E+ P is fast compared to ES -->E+S k2<<k-1 this statement cannot be completed because Km can never approximate Ks 2. Muscle contraction is triggered: by conformational change in myosin and actin in response to an increase in the cytoplasmic Ca2+ concentration in response to an increase in the cytoplasmic...
4. Km, the Michaelis-Menten constant is a composite of three rate constants. What are the three...
4. Km, the Michaelis-Menten constant is a composite of three rate constants. What are the three rate constants (in proper units)? What are the units of Km If enzyme A has a Km of ImM and enzyme B, a Km of 2 mM for the same substrate, which one is more efficient?
1. Define Vmax and Km (Michaelis constant). What is the mathematical relationship between these two values?...
1. Define Vmax and Km (Michaelis constant). What is the mathematical relationship between these two values? 2.What does Km tell you about the affinity of an enzyme for its substrate? 3.Why do we use the slope between the first two data points of Absorbance vs Time to determine V0 when studying enzyme kinetics? Why not use the slope of the entire line?
2) (5 marks) The enzyme Happyase follows simple Michaelis – Menten Kinetics a. The Km of...
2) (5 marks) The enzyme Happyase follows simple Michaelis – Menten Kinetics a. The Km of Happyase for its substrate ICE is Km^s = 1mM. Happyase also acts on substrate CREAM and its Km^T =10mM. Is ICE or CREAM the preferred substrate for Happyase? Explain b. The rate constant k2 with substrate ICE is 2x10^4sec^ -1; with substrate CREAM, k2=4x10^5sec ^-1. Does Happyase use substrate ICE or substrate CREAM with greater catalytic efficiency? Show calculations and explain your answer
2) (5 marks) The enzyme Happyase follows simple Michaelis – Menten Kinetics a. The Km of...
2) (5 marks) The enzyme Happyase follows simple Michaelis – Menten Kinetics a. The Km of Happyase for its substrate ICE is Kms = 1mM. Happyase also acts on substrate CREAM and its Km1 =10mM. Is ICE or CREAM the preferred substrate for Happyase? Explain b. The rate constant k2 with substrate ICE is 2x104sec-1; with substrate CREAM, k2=4x105sec-1. Does Happyase use substrate ICE or substrate CREAM with greater catalytic efficiency? Show calculations and explain your answer.
Ks is the dissociation constant Note: Numbers without units are often meaningless. 1. The maximum possible...
Ks is the dissociation
constant
Note: Numbers without units are often meaningless. 1. The maximum possible rate for a reaction between two molecules occurs when every collision results in reaction. In this case the rate is predicted to be k = (472) (D2+D2)r12 with units of Mi's! Here N is Avagadro's number (6.023 x 1023 molecules/mole), r12 is the critical distance for reaction, and D, and D2 are the diffusion coefficients for the two participants. In a typical enzyme catalyzed...
michaelis menten and kinetics help 2. A few years after Michaelis Menten published their work Briggs and Haldane came along and expanded it using the steady state assumption. i) Based on kinetic s...
michaelis menten and kinetics help
2. A few years after Michaelis Menten published their work Briggs and Haldane came along and expanded it using the steady state assumption. i) Based on kinetic scheme 2 how many different ways can ES be produced? i) Using the rate constants in kinetic Scheme 2 at what rate is the ES complex being produced? sed eci iv) Using the rate constants in kinetic Scheme 2 at what rate is the ES complex being destroyed?...
how we can solve q2 2. a) The Michaelis-Menten mechanism is +KTERE] - @s→Es (rateco nstant...
how we can solve q2
2. a) The Michaelis-Menten mechanism is +KTERE] - @s→Es (rateco nstant kl) ク ES→ E + S (rate constant k2) E S ES-XⓟHE) orate constant k3) So d[PVdt- k3[ES] Use the steady state approximation to show [El/[ES] (k2+k3)/(k1[S] b) let Km=(k2+k3)/kl and show that you get the expression ·J [EVIES]-Km/[S] c) We will talk in class about how this information eventually gives rise the expression d[P]/dt-k3E][S/(Km +IS) Usually [S>>Km. Show what this equation simplifies to...
4.4 For the enzyme reaction:The Michaelis and Menten constant, Km is actually a summary of 3 terms:(a) What are these? (b) What assumption does it make?(c) Define and calculate Km from the above equation given:(d) Which is the rate-limiting step? And why?
The Km can be considered to be the same as the dissociation constant K for E+ S binding it: the concentration of [ES] is unchanged k1 >> K2 ES --> E+ P is fast compared to ES -->E+S k2<<k-1 this statement cannot be completed because Km can never approximate Ks 2. Muscle contraction is triggered: by conformational change in myosin and actin in response to an increase in the cytoplasmic Ca2+ concentration in response to an increase in the cytoplasmic...
4. Km, the Michaelis-Menten constant is a composite of three rate constants. What are the three rate constants (in proper units)? What are the units of Km If enzyme A has a Km of ImM and enzyme B, a Km of 2 mM for the same substrate, which one is more efficient?
2) (5 marks) The enzyme Happyase follows simple Michaelis – Menten Kinetics a. The Km of Happyase for its substrate ICE is Kms = 1mM. Happyase also acts on substrate CREAM and its Km1 =10mM. Is ICE or CREAM the preferred substrate for Happyase? Explain b. The rate constant k2 with substrate ICE is 2x104sec-1; with substrate CREAM, k2=4x105sec-1. Does Happyase use substrate ICE or substrate CREAM with greater catalytic efficiency? Show calculations and explain your answer.
Ks is the dissociation
constant
Note: Numbers without units are often meaningless. 1. The maximum possible rate for a reaction between two molecules occurs when every collision results in reaction. In this case the rate is predicted to be k = (472) (D2+D2)r12 with units of Mi's! Here N is Avagadro's number (6.023 x 1023 molecules/mole), r12 is the critical distance for reaction, and D, and D2 are the diffusion coefficients for the two participants. In a typical enzyme catalyzed...
michaelis menten and kinetics help
2. A few years after Michaelis Menten published their work Briggs and Haldane came along and expanded it using the steady state assumption. i) Based on kinetic scheme 2 how many different ways can ES be produced? i) Using the rate constants in kinetic Scheme 2 at what rate is the ES complex being produced? sed eci iv) Using the rate constants in kinetic Scheme 2 at what rate is the ES complex being destroyed?...
how we can solve q2
2. a) The Michaelis-Menten mechanism is +KTERE] - @s→Es (rateco nstant kl) ク ES→ E + S (rate constant k2) E S ES-XⓟHE) orate constant k3) So d[PVdt- k3[ES] Use the steady state approximation to show [El/[ES] (k2+k3)/(k1[S] b) let Km=(k2+k3)/kl and show that you get the expression ·J [EVIES]-Km/[S] c) We will talk in class about how this information eventually gives rise the expression d[P]/dt-k3E][S/(Km +IS) Usually [S>>Km. Show what this equation simplifies to...
Most questions answered within 3 hours.
-
Modify
When executing on the command line having only
this program name, the program will accept...
asked 57 seconds ago -
Kenny Electric Company's noncallable bonds were issued several
years ago and now have 20 years to...
asked 16 minutes ago -
find H(e^Jtheta) at theta= 0, pi/10, pi/20, pi/2 for
the following:
a) H(e^Jtheta)= 1+e^Jtheta
b) H(e^Jtheta)=...
asked 45 minutes ago -
Home Corporation will open a new store on January 1. Based on
experience from its other...
asked 1 hour ago -
In a neoclassical model, use the IS-LM to analyze the effect of
a permanent money supply...
asked 1 hour ago -
An electron passes through a point 2.67 cm from a long straight
wire as it moves...
asked 2 hours ago -
A grammar is a 4-tuple G, G = (Ν, Σ, Π, Σ, S) where, Ν is...
asked 2 hours ago -
In this part, calculate the present values. Use the Excel PV
function to compute the present...
asked 2 hours ago -
Part 1. Primitive Types, Sorting, Recursion for
Homework.java
a) Implement the static method initializeArray that receives...
asked 4 hours ago -
Using C++, build a sorter that can rank a sequence of numbers in
a descending order....
asked 3 hours ago -
Derive ground state term symbols. Use notation 2S(1/2) for state
2S1/2
a) d5
b) f3
c)...
asked 4 hours ago -
A sample of size 31 will be drawn from a population with mean 39
and standard...
asked 5 hours ago