Question

2. A few years after Michaelis Menten published their work Briggs and Haldane came along and expanded it using the steady state assumption. i) Based on kinetic scheme 2 how many different ways can ES be produced? i) Using the rate constants in kinetic Scheme 2 at what rate is the ES complex being produced? sed eci iv) Using the rate constants in kinetic Scheme 2 at what rate is the ES complex being destroyed? v) The steady state assumptions assumes that the ES complex is being destroyed and produced at the same rate (d[ES]/dt 0)so please set the result from parts i) and iv) to equal each other. Plug in [E]T -[ES] for [E] and solve for [ES] in terms of [E]r IS], ki, k1, and k2 vi) What is the rate at which ES reacts to produce P (i.e. d[P]/dt)? vil) Based on your answer in part vi what is the rate at which product is produced in terms of [Ehr [S], KM. and k2. Assume KM (kitkJ/ki welcome to the MM equation.

michaelis menten and kinetics help

Answer 1

Leat LD a). t bindc to S b) Es eacts to secand ubetrata to gene es Rate of formabon of e

Cec D 2 Relative to total CETJ Ctcy EsJ d [ts] ESS d t ti) s can dectroyed by - a). dissouaton into E and s b). convergion ent Parod uet" iv) .91 cvell depend on keat 약,8E and KD1kkD2 dt CECT dlt

dt ES へcat dt Relati'vet total e c) KDL KD1 D 2 LESSJ 2 KDI KD1 KDn ve). ko KL