Question

For live cell imaging, the PBS solution often contains 0.9 mM calcium chloride (molecular weight = 110.98) and 0.49 mM magnesium chloride hexahydrate (molecular weight = 203.3). For 1 liter of PBS, how much calcium chloride and magnesium chloride would you have to add to the standard PBS recipe? (Answer to 2 decimal places)

Answer 1

A caelz = 0.9mm -Macl = 0.49mm 12 0.0998 g = 0.09g cach = 140.9=0.9 minoles = 0.98 110.98 ing - 99.882ing = Mgch - 1x0.49=0.49 mindles Mach. (H2O=409 0.4aunches = 0.49x 203.3 mg - 99.617ing = 0.09969 -0.09 g