Radioactive 32P is an isotope commonly used to study DNA replication and repair. Incorporation of the isotope occurs through modified dNTPs. If the 32P isotope is introduced into the β phosphate of an dNTP would you expect to see radioactive DNA daughter strands during replication? What about the α phosphate? Why?
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Radioactive 32P is an isotope commonly used to study DNA replication and repair. Incorporation of the...
Please need help answering question A the pages of background information are posted thanks Read page 196-197 and figure 6.20. regarding Meselson and Stahl’s experiment regarding DNA replication. And Answer the following question If you are using this radioactive technique in mouse cells, what would happen in each phase of G1, S, G2, mitosis and meiosis assuming that you are grown cells in 15N medium for many generations and cells in G1are then switched to 14N medium? G1 S G2...
Unit 3 Study Resource Meiosis • Process by which diploid cells create haploid cells NOT part of the cell cycle > only some cells ever undergo meiosis During meiosis I, homologous chromosomes line up to allow them to be separated into two new cells o They can become "tangled" during this phase, which leads to crossing-over (rearranging the alleles) O Result of meiosis I is two non-identical haploid cells Meiosis Il looks very similar to mitosis, in that sister chromatids...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
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10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated sludge operation that adversely affect effluent quality with origins in the engineering, hydraulic and microbiological components of the process. The real "heart" of the activated sludge system is the development and maintenance of a mixed microbial culture (activated sludge) that treats wastewater and which can be managed. One definition of a wastewater treatment plant operator is a "bug farmer", one who controls the aeration...