Explain in general the steps and materials necessary to perform biochemical and enzymatic tests for microbial...
Explain in general the steps and materials necessary to perform biochemical and enzymatic tests for microbial identification
Homework Answers
Test for microbial identification by catalase enzyme test :
Microorganism that live in oxygenated environment is aerobes neutralize the toxic form of oxygen metabolites hydrogen peroxide to break down into hydrogen and oxygen which is done by catalase enzyme present in aerobic bacteria.
This test is responded by aerobic bacteria like staphylococcus and it gives negative result in case of anaerobic bacteria
Material: A clean glass slide, 3% hydrogen peroxide and a sterile loop
Procedure:
- The small amount of bacterial colony is taken in a sterilized slide with a sterilized loop.
- Small amount of 3% hydrogen peroxide is mixed with it.
- Disposed the slide in a biohazard glass disposal container
Observation: The bubble is formed in 5 to 10 seconds
Results: The bubble is formed due to present of aerobic bacteria which breaks down hydrogen peroxide to form oxygen.
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