Based on the discussed protein purification/characterization techniques which chain of experiments makes logical sense if one...
Based on the discussed protein purification/characterization techniques which chain of experiments makes logical sense if one wants to study protein structure?
| a. |
Cell/tissue homogenization->Affinity chromatography->Mass-Spec-> Gel filtration chromatography |
|
| b. |
Cell/tissue homogenization->Mass-Spec->Gel filtration chromatography->Affinity chromatography |
|
| c. |
Cell/tissue homogenization->Gel filtration chromatography->Affinity chromatography->Mass-Spec |
|
| d. |
Gel filtration chromatography->Cell/tissue homogenization->Affinity chromatography->Mass-Spec |
Homework Answers
Answer: C: cell/tissue homogenization > Gel filtration chromatography > Affinity chromatography > Mass-spec
Explanation:
Prior to protein purification, the cell or tissue should be homogenized by disrupting the cell membrane to obtain a crude extract of cellular components, proteins and the nucleic acids. The cell or tissue homogenate contains different proteins of different size and shape, so the proteins can be separated based on the size using gel filtration chromatography. Further, a specific protein is purified using affinity chromatography. Affinity chromatography separates the protein of interest based on its ability to bind specifically to another molecule. Hence, the specific protein of interest can be isolated from the protein extract using affinity chromatography. Further, the mass spectrometry is used to study the structure of the protein.
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Based on the discussed protein
purification/characterization techniques which chain of experiments
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